Preliminary evaluation of human hair follicle-derived dermal papilla cell characteristics

Authors

  • Dao Thi Thanh Thuy
    University of Science, Ho Chi Minh City Vietnam National University, Ho Chi Minh City, VN
  • Phan Van Tien
    University of Science, Ho Chi Minh City Vietnam National University, Ho Chi Minh City, VN
  • Van Duc Huy
    University of Science, Ho Chi Minh City Vietnam National University, Ho Chi Minh City, VN
  • Nguyen Thi Ngoc Nhu
    University of Science, Ho Chi Minh City Vietnam National University, Ho Chi Minh City, VN
  • Phan Lu Chinh Nhan
    University of Science, Ho Chi Minh City Vietnam National University, Ho Chi Minh City, VN
  • Nguyen Ton Ngoc Huynh
    DNA International Hospital, Ho Chi Minh City, VN
  • Dang Thi Tung Loan
    University of Science, Ho Chi Minh City Vietnam National University, Ho Chi Minh City, VN

DOI:

10.46223/HCMCOUJS.tech.en.13.2.2801.2023

Keywords:

characteristic; dermal papilla; fibroblast; hair follicle; human

Abstract

Dermal Papilla Cells (DPCs) are mesenchymal cells residing at the base of the hair follicles. They play an important role in the hair cycle remodeling and hair follicle regeneration. In this study, DPCs were isolated, and cultured from human hair follicles and evaluated the expression of -SMA, versican by immunocytochemistry, and alkaline phosphatase activity. The human fibroblasts were used as a control to confirm the non-fibroblast DPCs. The results showed that the cells grew from the hair bulb and had fibroblast morphology. These bulb cells strongly expressed alkaline phosphatase activity -SMA, and versican while the human fibroblast lacked ALP activity and less expression of -SMA and versican. In conclusion, the protocol is established to isolate and culture DPCs from hair follicle bulbs, and ALP activity is suggested as the major marker for distinguishing DPCs and human fibroblasts.

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References

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Received: 05-06-2023
Accepted: 13-06-2023
Published: 31-10-2023

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How to Cite

Thuy, D. T. T., Tien, P. V., Huy, V. D., Nhu, N. T. N., Nhan, P. L. C., Huynh, N. T. N., & Loan, D. T. T. (2023). Preliminary evaluation of human hair follicle-derived dermal papilla cell characteristics. HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY, 13(2), 3–11. https://doi.org/10.46223/HCMCOUJS.tech.en.13.2.2801.2023

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Copyright (c) 2023 Dao Thi Thanh Thuy; Phan Van Tien; Van Duc Huy; Nguyen Thi Ngoc Nhu; Phan Lu Chinh Nhan; Nguyen Ton Ngoc Huynh; Dang Thi Tung Loan

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.