--

11 (1) 2021

Direct embryogenesis from anther culture of hot chilli Capsicum annuum L.


Author - Affiliation:
Nguyen Tran Dong Phuong - Ho Chi Minh City Open University, Vietnam
Corresponding author: phuong.ntd@ou.edu.vn
DOI:10.46223/HCMCOUJS.tech.en.11.1.560.2021

Abstract
In Viet Nam, local varieties of chili have a distinctive aroma and pungency. However, the generation of pure lines from pollen culture in local hot chilli has been very limited reported. Therefore, this study was to relate flower bud size with microspore developmental stages and the culture media have concentration changed of growth regulator effects on the in vitro androgenesis. Flower buds were randomly collected and visually divided into three stage based on both petal and sepal size. Then anther was cultured on MS basal medium with different concentration of hormones NAA changed 0.1 - 0.7 mg/L and kinetin changed 1.0 - 3.0 mg/L, BA changed 0.5 - 1.5 mg/L. The results showed that bud flower have anthers are light violet in color, 2.5 mm long, consisted of anthers with 80 % uni-nucleate and 20 % bi-nucleate microspores were selected. In induction culture media, it was observed that MS medium with 2.0 mg/L Kinetin and 0.5 mg/L NAA gave higher embryo frequency. MS medium with 1 mg/L BA is the best medium for embryo germination and inducting shoots. And ½ MS medium for shoot elongation and rooting.

Keywords
androgenesis;antherculture; culture media direct embryogenesis; haploid; hot chilli

Full Text:
PDF

References

Arjunappa, H. M., Kumar, S., & Latha, P. (2016). Effect of genotype and media on direct embryogenesis of chilli pepper (Capsicum annuum L.). International Journal of Recent Scientific Research, 7(1), 8592-8595.


Ciner, D. O., & Tipirdamaz, R. (2002). The effects of cold treatment and charcoal on the in vitro androgenesis of pepper (Capsicum annuum L.). Turkish Journal of Botany, 26, 131-139.


Lantos, C., Juhasz, A. G., Vagi, P., Mihaly, R., Kristof, Z., & Paul, J. (2012). Androgenesis induction in microspore culture of sweet pepper (Capsicum annuum L.). Plant Biotechnology Reports, 6, 123-132.


Nowaczyk, P., & Kisiala, A. (2006). Effect of selected factors on the effectiveness of Capsicum annuum L. anther culture. Journal of Applied Genetics, 47(2), 113-117.


Rodeva, V. N., Irikova, T. P., & Todorova, V. J. (2011). Anther culture of pepper (Capsicum Annuum L.): Comparative study on effect of the genotype. Biotechnology & Biotechnological Equipment, 18(3), 34-38.


Vaulx, D. R., Chambonnet, D., & Pochard, E. (1981). In vitro culture of pepper (Capsicum annuum L.) anthers: High rate plant production from different genotypes by +35 °C treatments. Agronomie, 1, 859-864.


Yang, B. Z., Zhou, S. D., Zhang, Z. Q., Dai, X. Z., Li, L. H., & Xie, D. P. (2009). Effects of different medium and hormone on cultured anther of hot pepper. Journal of Hunan Agricultural University, 35(1), 61-64.



View Counter: Abstract | 5 | times
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.