Evaluating the potential anti-Methicillin-Resistant Staphylococcus Aureus (MRSA) activities from extracts of Eleutherine subaphylla Gagnep

Abstract

Methicillin-Resistant Staphylococcus Aureus (MRSA) is a significant cause of infections in hospitals and communities worldwide. Staphylococcus aureus is classified as a high-alert antimicrobial-resistant organism. Currently, alongside advancements in medicine, naturally occurring compounds with bioactive properties have garnered the attention of the scientific community due to their safety, low side effects, and potential as sustainable, promising alternatives. This study evaluated the antimicrobial activity of extracts of Eleutherine subaphylla Gagnep, against Methicillin-Resistant Staphylococcus Aureus (MRSA). The results indicated that the n-hexane extract exhibited the highest antibacterial efficacy against MRSA (28.00 ± 0.86mm). The n-hexane extract demonstrated a Minimum Inhibitory Concentration (MIC) of 31.25 µg/mL against MRSA. By combining column and thin-layer chromatography, a crystalline compound, ES02, was isolated and purified, demonstrating the most substantial resistance to MRSA. Based on ¹H-NMR spectral data, the structure of compound ES02 was identified as eleutherol. The results of this study reported bioactive compounds from Eleutherine subaphylla Gagnep. had the potential to be further applied against antibiotic-resistant bacteria.