Bioinspired synthesis of Ag/AgCl nanoparticles from  Aganonerion Polymorphum leaves: boosted antibacterial potential

Thanh Gia Thien Ho; Minh Van Nguyen; Linh Nhat Duong; Long Ba Do; Anh Phung Nguyen; Tri Nguyen

doi:10.46223/HCMCOUJS.tech.en.15.2.4228.2025

15(2)2025 (IN PRESS)

Bioinspired synthesis of Ag/AgCl nanoparticles from Aganonerion Polymorphum leaves: boosted antibacterial potential

Author - Affiliation:

Thanh Gia Thien Ho - Vietnam Academy of Science and Technology, Ho Chi Minh City , Vietnam

Minh Van Nguyen - Ho Chi Minh City Open University, Ho Chi Minh City , Vietnam

Linh Nhat Duong - Ho Chi Minh City Open University, Ho Chi Minh City , Vietnam

Long Ba Do - Vietnam Academy of Science and Technology, Ho Chi Minh , Vietnam

Anh Phung Nguyen - Vietnam Academy of Science and Technology, Ho Chi Minh , Vietnam

Tri Nguyen - Vietnam Academy of Science and Technology, Ho Chi Minh , Vietnam

Corresponding author: Tri Nguyen - ntri@ict.vast.vn

DOI:10.46223/HCMCOUJS.tech.en.15.2.4228.2025

Submitted: 18-03-2025
Accepted: 02-05-2025
Published: 06-05-2025

Abstract

The use of plant-extracts (mainly polyphenols) as both reducing and stabilizing agents has opened up new scopes in AgNPs biosynthesis. A lack of comprehensive studies on possibility of the Aganonerion polymorphum (AP) leaves extract prompts an update synthesis of Ag/AgCl nanoparticles thanks to a rich source of chlorine of the AP leaves extract, in order to illumine the positive effect of AgCl in antibacterial systems. Ag/AgCl nanoparticles were efficiently synthesized using AP leaf extract, serving as both a natural reducing and stabilizing agent. In fact, the highest concentrations of polyphenols (2.55 mg/g) and ascorbic acid (2.89 µg/mL) were extracted under optimal conditions (at 70 °C for 1.5 hours), acting as the phytochemicals for metal ion reduction to form Ag/AgCl nanoparticles under sunlight, observed by absorbance bands at the wavelength of 400500 nm. XRD showed a highly crystalline face-centered cubic structure of Ag and AgCl, and EDX indicated the presence of Ag and Cl. TEM revealed spheres with nano-size of 1520 nm. The zeta potential confirmed the long-term stability of the Ag/AgCl in aqueous solution (8.7 mV). As-prepared Ag/AgCl demonstrated an excellent antibacterial ability against five bacterial via their inhibition zone (average diameter >9.3 mm), and minimum inhibitory concentration (MIC, 16.6 μg/mL). This study proposes a green and sustainable strategy for synthesizing Ag/AgCl nanoparticles, employing AP leaf extract as a natural reducing and stabilizing agent under ambient temperature conditions. The synthesized Ag/AgCl nanoparticles exhibited potent antibacterial activity against both Gram-positive and Gram-negative bacteria. Notably, the presence of AgCl further enhanced their efficacy, expanding their potential for advanced antibacterial applications.

Keywords

Aganonerion polymorphum; ascorbic acid; antibacterial; polyphenols; silver nanoparticles

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DOI: https://doi.org/10.46223/HCMCOUJS.tech.en.15.2.4228.2025