Isolation and identification of some . Strain from traditional fermented foods

Truong Kim Phuong; Nguyen Trong Nghia; Le Huyen Ai Thuy

5 (1) 2015

Isolation and identification of some . Strain from traditional fermented foods

Author - Affiliation:

Truong Kim Phuong - Ho Chi Minh City Open University , Vietnam

Nguyen Trong Nghia - Ho Chi Minh City Open University , Vietnam

Le Huyen Ai Thuy - Ho Chi Minh City Open University , Vietnam

Corresponding author: Le Huyen Ai Thuy - thuy.lha@ou.edu.vn

Abstract

Recent publications showed that Lactic acid bacteria (LAB) are used extensively to inhibit growth of spoilage and pathogenic bacterial strains. That is being applied in food processing such as tradition fermented food or dairy, beverage and meat products. Lactic acid bacteria can produce a variety of antibacterial agents including bacteriocin, diacetyl, etc. Thus, the isolation, identification and taxonomical characterization of each new Lactobacillus sp. strain is being more and more required. However, the large number of species in the genus Lactobacillus almost have their high phenotypic and physiological similarity which easily leads to misidentification.
The present study was aimed for isolation and reliable identification of Lactobacillus sp. strains from some traditional fermented foods by on the basis of phenotypic analysis and combination of PCR and sequencing of target sequences base on 16S-23S rRNA gene. Eight strains of LAB were isolated and characterized through morpholigical, physiological, biochemical and carbohydrate fermentation tests. All of them were determined as Lactobacillus sp. Moreover, the nucleotides sequences of 16S-23S rDNA of them was compared and phylogenetic analysis to those of Lactosbacillus species in GenBank and the results confirm that four strains: L1, L3, L4 and L7 belong to Lactobacillus platarum and four strains: L2, L5, L6 and L8 belong to species L. rhamnosus

Keywords

Lactic acid bacteria (LAB); Lactobacillus platarum; 16S-23S rRNA inter spacer region

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References

Amann R.I., Ludwig W., Schleifer K.-H. (1995). Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiological Reviews, Vol 59(1), 143–169.

Ampe F., ben Omar N., Guyot J.P. (1999). Culture-independent quantification of

physiologically-active microbial groups in fermented foods using rRNA-targeted oligonucleotide probes: application to pozol, a Mexican lactic acid fermented maize dough. Journal of Applied Microbiology, Vol 87(1), 131-140.

Beste A. C., Logan A. C., Selhub E. M. (2013). Intestinal microbiota, probiotics and mental health: from Metchnikoff to modern advances: Part II – contemporary contextual research, Gut Pathogens, Vol 5(1), 3.

Bixquert Jiménez M. (Aug 2009). Treatment of irritable bowel syndrome with probiotics. An etiopathogenic approach at last?. Revista espanola de enfermedades digestivas: organo oficial de la Sociedad Espanola de Patologia Digestiva, Vol 101(8), 553–564.

Case R. J., Boucher Y., Dahllöf I., Holmström C., Doolittle W. F., Kjelleberg S. (2007). Use of 16S rRNA and rpoB Genes as Molecular Markers for Microbial Ecology Studies, Applied and Environmental. Microbiology, Vol 73 (1), 278–288.

Clarridge J. E. (2004). Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clinical Microbiology Reviews, Vol 17 (4), 840–862.

Coeuret V., Dubernet S., Bernardeau M., Gueguen M., Vernoux J. P. (2003). Isolation, characterisation and identification of Lactobacilli focusing mainly on cheeses and other dairy products. Lait, Vol. 83(4), 269-306.

Gürtler V., Stanisich V. (1996). New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region. Microbiology, Vol 142( Pt 1), 3-16.

Hamad S. H., Dieng M. C., Ehrmann M. A., Vogel R. F. (1997). Characterization of the bacterial flora of sudaneseSudanese sorghum flour and sorghum sourdough. Journal of Applied Microbiology, Vol 83(6), 764-770.

Hammes W. P., Vogel. R. F. (1995). The genus Lactobacillus. In Wood B. J. B. and Holzapfel W. H. (ed.). The genera of lactic acid bacteria. Blackie Academic and Professional, London, United Kingdom, Vol 2, 19–54.

Holzapfel W. H., Haberer P., Geisen R., Björkroth J., Schillinger U. (2001). Taxonomy and important features of probiotic microorganisms in food and nutrition. American Journal of Clinical Nutrition, Vol. 73(2), 365-373.

Kandler O., Weiss N. (1986). Regular, nonsporing gram-positive rods. In Sneath P. H. A. (ed.). Bergey's Manual of Systematic Bacteriology. Williams and Wilkins, Baltimore, Md.,, Vol 2, 1209-1234.

Ki M. R., Lee S. J., Seul K. J., Park Y. M., Ghim S. Y. (2009). Characterizaton of antimicrobial substance produced by Lactobacillus paraplantarum KNUC25 isolated from kimchi, Korea Journal of Microbiology Biotechnology, Vol. 37(1), 24-32.

Klein G., Pack A., Bonaparte C., Reuter G. (1998). Taxonomy and physiology of probiotic lactic acid bacteria, International Journal of Food Microbiology, Vol. 41(2), 103-125.

Kolbert C. P., Persing D. H. (1999). Ribosomal DNA sequencing as a tool for identification of bacterial pathogens, Current Opinion in Microbiology, Vol 2(3), 299–305.

Kwon H. S., Yang E. H., Yeon S. W., Kang B. H., Kim T. Y. (2004). Rapid identification of probiotic Lactobacillus species by multiplex PCR using species-specific primers based on the region extending from 16S rRNA through 23S rRNA, FEMS Microbiology Letters, Vol 239, 267–275.

Markiewicz L., Biedrzycka E. (2005). Identification of Lactobacillus and Bifidobacterium species with PCR applied to quality control of fermented dairy beverages. Polish Journal of Food And Nutrition Sciences, Vol 14/55 (4), 359-366.

Müller M. R. A., Ehrmann M. A., Vogel R. F. (2000). Multiplex PCR for the detection of Lactobacillus pontis and two related species in a sourdough fermentation. Applied and Environmental Microbiology, Vol 66(5), 2113–2116.

Pereira F., Carneiro J., Matthiesen R., van Asch B., Pinto N., Gusmao L., Amorim A. (2010). Identification of species by multiplex analysis of variable-length sequences, Nucleic Acids Research, Vol 38(22), e203–e203.

Rosselló-Mora R., Amann R. (2001). The species concept for prokaryotes. FEMS Microbiology Review, Vol 25(1), 39–67.

Sankar N. R., Priyanka V. D., Reddy P. S., Rajanikanth P., Kumar V. K., Indira M. (2012). Purification and characterization of bacteriocin produced by Lactobacillus plantarum isolated from cow milk, International Journal of Microbiological Research, Vol 3(2), 133-137.

Song Y., Kato N., Liu C., Matsumiya Y., Kato H., Watanabe K. (2000). Rapid identification of 11 human intestinal Lactobacillus species by multiplex PCR assays using group- and species-specific primers derived from the 16S-23S rRNA intergenic spacer region and its flanking 23S rRNA. FEMS Microbiology Letters, Vol 187(2), 167-173.

Torriani S., van Reenen C. A., Klein G., Reuter G., Dellaglio F., Dicks L. M. T. (1996). Lactobacillus curvatus subsp. curvatus subsp. nov. and Lactobacillus curvatus subsp. melibiosus subsp. nov. and Lactobacillus sake subsp. sake subsp. nov. and Lactobacillus sake subsp. carnosus subsp. nov., new subspecies of Lactobacillus curvatus Abo-Elnaga and Kandler 1965 and Lactobacillus sake Katagiri, Kitahara, and Fukami 1934 (Klein et al. 1996, emended descriptions), respectively, International Journal of Sysmatic Bacteriology, Vol 46 (4), 1158-1163.

Vandamme P., Pot B., Gillis M., de Vos P., Kersters K., Swings J. (1996). Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Reviews, Vol 60(2), 407–438.

Woese, C. R, Fox G. E. (1977). Phylogenetic structure of the prokaryotic domain: The primary kingdoms, Proceedings of the National Academy of Sciences, 74 (11), 5088–5090

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