--

5 (1) 2015

Discorvery of Entomopathogenic fungi Cordyceps takaomontana at Langbian Mountain, Lam Dong, Vietnam


Author - Affiliation:
Vu Tien Luyen - University of Science, VNU-HCM , Vietnam
Trinh Van Hanh - Ho Chi Minh City Open University , Vietnam
Trinh Hoang Luan - Ho Chi Minh City Open University , Vietnam
Nguyen Thi Bich Thao - Ho Chi Minh City Open University , Vietnam
Dinh Minh Hiep - Agricultural Hi-tech Park, Vietnam , Vietnam
Truong Binh Nguyen - University of Da Lat, Vietnam , Vietnam
Lao Duc Thuan - Ho Chi Minh City Open University , Vietnam
Corresponding author: Lao Duc Thuan - thuan.ld@ou.edu.vn

Abstract
The stromata of Cordyceps takaomontana Y. Kobayashi (1941) were found on the Langbian Mountain, Da Lat, Vietnam at the height of 1.650 meter above sea level, on the larva of Lepidoptera. Stromata were lemon-yellow, clavate to enlongated clavate, arising from a white pseudosclerotium. The fertile head was on the top part of stromata, darker colored in comparison to the stipe. Perithecium was narrowly ovoid, superficial and forming dark yellow punctate on the surface of stromata. Ascus was cylindrical with semi-spherical cap. Ascospores were cylindrical, truncated and separately after discharge from the ascus.
Pure culture was isolated on Potato Glucose Agar (PGA) medium: white colony in young and yellow in old. The isolated mycelium was not homogenous in thickness and in growth rate at the peripheral area. Conidiophores were phialide, tapering to both apexes. Conidia had elliptical shape and formed into chains after maturation.
DNA was isolated, then purified from pure mycelium and used to amplifying the nrLSU (nuclear ribosomal large subunit) and rpb1 (RNA polymerase II largest subunit) genes. The amplified products were used for sequencing, proof-reading by some professional softwares before combining with other nrLSU and rpb1 sequences. Then this database was used to search for the suitable evolution model as well as to construct the phylogenetic trees.
The results of phylogenetic analysis completely supported the morphological classification: DL0038A and DL0038B were Cordyceps takaomontana Y. Kobayashi (1941).

Keywords
Cordyceps takaomontana; Langbian; nrLSU; rpb1; Kobayashi.

Full Text:
PDF

References

Altschul S. F., Gish W., Miller W., Myers E. W. and Lipman D. J. (1990). Basic local alignment search tool. J Mol Biol, 215, 403-410.


Chomczynski P. and Sacchi N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem, 162, 156-159.


Darriba D., Taboada G. L., Doallo R and Posada D.J. (2012). ModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9(8), 772.


Dentinger B. T. and McLaughlin D. J. (2006). Reconstructing the Clavariaceae using nuclear large subunit rDNA sequences and a new genus segregated from Clavaria. Mycologia, 98(5), 746-762.


Gouy M., Guindon S. and Gascuel O. (2010). SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Molecular Biology and Evolution, 27, 221-224.


Hodge K. T., Krasnoff S. B. and Humber R. A. (1996). Tolypocladium inflatum is the anamorph of Cordyceps subsessilis. Mycologia, 88(5), 715-719.


Kobayasi Y. (1982). Keys to the taxa of the genera Cordyceps and Torrubiella.


Transactions of the Mycological Society of Japan, 23, 329-54.


Liang Z.Q., Liu A.Y., Liu M.H and Kang J.C. (2003). The genus Cordyceps and its allies from the Kuankoushui Reserve in Guizhou III. Fungal Diversity 14, 95-101.


Luangsa-ard J.J., Tasanathai K., Mongkolsamrit S and Hywel-Jones N. (2007). Atlats of Invertebrate-Pathogenic Fungi of Thailand. Biotec. NSTDA Thailand.


Mains E. B. (1957). Species of Cordyceps parasitic on Elaphomyces. Bulletin of the Torrey Botanical Club, 84, 243-251.


Reiss E., Shadomy H. J. and Lyon G. M. (2011). Fundamental Medical Mycology. WileyBlackwell, 64-65.


Sonnenberg R., Nolte W. and Tautz D. (2007). An evaluation of LSU rDNA D1-D2 sequences for their use in species identification. Front Zoo,4, 6.


Stiller JW, Hall BD. (1997). The origin of red algae: Implications for plastid evolution. Proc. Natl. Acad. Sci, 94, 4520 – 4525.


Sung G. H., Hywel-Jones N. L., Sung J. M., Luangsa-ard J. J., Shrestha B. and Spataforal J. W. (2007). Phylogenetic classification of Cordyceps and the Clavicipitaceous fungi. Stud Mycol, 57, 5-59.


Tamura K., Stecher G., Peterson D., Filipski A. and Kumar S. (2013). MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol, 30(12), 2725-2729.


Technelysium South Brisbane. Chromas Pro phiên bản 1.7.4 QLD, Australia (2003-2012).


White T. J., Bruns T., Lee, S. and Taylor J. (1990). In PCR Protocols: A Guide to Methods and Applications. Academic Press, 315-322.



Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.