Discorvery of Entomopathogenic fungi Cordyceps takaomontana at Langbian Mountain, Lam Dong, Vietnam

Abstract

The stromata of Cordyceps takaomontana Y. Kobayashi (1941) were found on the Langbian Mountain, Da Lat, Vietnam at the height of 1.650 meter above sea level, on the larva of Lepidoptera. Stromata were lemon-yellow, clavate to enlongated clavate, arising from a white pseudosclerotium. The fertile head was on the top part of stromata, darker colored in comparison to the stipe. Perithecium was narrowly ovoid, superficial and forming dark yellow punctate on the surface of stromata. Ascus was cylindrical with semi-spherical cap. Ascospores were cylindrical, truncated and separately after discharge from the ascus. Pure culture was isolated on Potato Glucose Agar (PGA) medium: white colony in young and yellow in old. The isolated mycelium was not homogenous in thickness and in growth rate at the peripheral area. Conidiophores were phialide, tapering to both apexes. Conidia had elliptical shape and formed into chains after maturation. DNA was isolated, then purified from pure mycelium and used to amplifying the nrLSU (nuclear ribosomal large subunit) and rpb1 (RNA polymerase II largest subunit) genes. The amplified products were used for sequencing, proof-reading by some professional softwares before combining with other nrLSU and rpb1 sequences. Then this database was used to search for the suitable evolution model as well as to construct the phylogenetic trees. The results of phylogenetic analysis completely supported the morphological classification: DL0038A and DL0038B were Cordyceps takaomontana Y. Kobayashi (1941).