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5 (1) 2015

The effect of paraquat herbicide on In Vivo spermatogenesis in mice


Author - Affiliation:
Nguyen Nhut Vu - Ho Chi Minh City Open University , Vietnam
Nguyen Thi Thuy Hang - Ho Chi Minh City Open University , Vietnam
Nguyen Thi Bich Thao - Ho Chi Minh City Open University , Vietnam
Trinh Huu Phuoc - Ho Chi Minh City Open University , Vietnam
Corresponding author: Trinh Huu Phuoc - trinhhuuphuoc.ou@gmail.com

Abstract
Paraquat (PQ) herbicide (1,1-dimethyl 4,4’-bipiridillium) is the most widely used in agricultural area on more than 100 crops in about 100 countries. PQ is highly acutely toxic with an acute oral LD50 for rat of 157 mg/kg. For aims to evaluate the toxic effects of low concentration of Paraquat on mouse spermatogenesis, mice (5 groups, n =7) were inter-dermal injected to the scrotal sac with the dose of PQ with four concentration as T1 (10 mg/kg), T2 (15mg/kg), T3 (20 mg/kg), T4 (25 mg/kg) and a control group with PBS treated. The evaluation of spermatogenesis were carried out by behavioral changes observation, weight body assessments, sperm counting and histological testis HE stained. The results indicated that PQ has toxic effects on spermatogenesis, especially, in the dose of 25 mg/kg, PQ significantly effect on the structureof testis leads to the failure of sperm producing.

Keywords
Paraquat; spermatogenesis; testes; sperm counting; HE staining.

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