The effect of paraquat herbicide on In Vivo spermatogenesis in mice

Abstract

Paraquat (PQ) herbicide (1,1-dimethyl 4,4’-bipiridillium) is the most widely used in agricultural area on more than 100 crops in about 100 countries. PQ is highly acutely toxic with an acute oral LD50 for rat of 157 mg/kg. For aims to evaluate the toxic effects of low concentration of Paraquat on mouse spermatogenesis, mice (5 groups, n =7) were inter-dermal injected to the scrotal sac with the dose of PQ with four concentration as T1 (10 mg/kg), T2 (15mg/kg), T3 (20 mg/kg), T4 (25 mg/kg) and a control group with PBS treated. The evaluation of spermatogenesis were carried out by behavioral changes observation, weight body assessments, sperm counting and histological testis HE stained. The results indicated that PQ has toxic effects on spermatogenesis, especially, in the dose of 25 mg/kg, PQ significantly effect on the structureof testis leads to the failure of sperm producing.