Rapid detection of betanodavirus presence in sea bass (lates calcrifer) based on RT-PCR (reverse transcriptase PCR)

Abstract

Betanodavirus are the causative agents of viral nervous necrosis (VNN) in cultured marine fish, as Sea bass (Lates calcarifer). The aims of current study toward in establishing the rapid method to detect the presence of Betanodavirus in Sea bass. A total of 30 death aquarium Sea bass, which were collected from Ben Tre province, Vung Tau province and positive control which confirmed within viral nervous necrosis, were enrolled into present study. Initially, we were successful in primers designed, which were also confirmed the specificity to Betanodavirus and sensitivity which reached at least 103 copies/ml. Finally, we successfully adopted rapid detection of Betanodavirus presence based on RT-PCR. As the results, 3 of 30 cases were positive to Betanodavirus. Then, the results were confirmed by sequencing and the similarity was showed. Therefore, in further study, it will be continuous in detecting the presence of Betanodavirus in various kinds of fishery samples.