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15(1)2025

Extraction process optimization for maximizing total polyphenol content in cashew nut testa (Anacardium occidentale L.) extract


Author - Affiliation:
Dung Thi Nguyen - Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City , Vietnam
Toan Van Nguyen - Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City , Vietnam
Tu Le Kha Bui - Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City , Vietnam
Lieu Thi Nguyen - Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City , Vietnam
Son Hai Pham - Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City , Vietnam
Trinh Thi Nu Nguyen - Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City , Vietnam
Trang Thi Thuy Nguyen - Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City , Vietnam
Thinh Bao Bui - Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City , Vietnam
Corresponding author: Thinh Bao Bui - buibaothinh9595@gmail.com
Submitted: 09-10-2024
Accepted: 19-11-2024
Published: 13-01-2025

Abstract
Cashew nut testa (Anacardium occidentale L.) is a valuable source of polyphenols known for their strong bioactivities. This study optimized the extraction conditions to obtain polyphenol-rich extracts from cashew nut testa. The effects of three key factors, extraction time, material-to-solvent ratio, and temperature, on Total Polyphenol Content (TPC, mg GAE/g extract) were investigated. Response surface methodology, using a Box-Behnken design, was applied to design the experiments and optimize the extraction process. The results showed that the relationship between TPC and extraction conditions followed a second-order model with an R² value of 0.9999. All three factors significantly influenced TPC (p < 0.05), and their interactions were also significant. The model predicted optimal conditions for maximum TPC at an extraction time of 19.85h, a temperature of 58.80°C, and a material-to-solvent ratio of 1:16.39 (w/v). Validation experiments under the optimized conditions of 20h, 59°C, and a 1:16 ratio confirmed the model's accuracy, yielding a TPC of 534.67mg GAE/g extract, statistically equivalent to the predicted value. These findings demonstrate the efficacy of the optimized conditions in maximizing polyphenol extraction from cashew nut testa.

Keywords
Anacardium occidentale; cashew nut testa; extraction conditions; polyphenol; response surface methodology

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Cite this paper as:

Nguyen, D. T., Nguyen, T. V., Bui, T. L. K., Nguyen, L. T., Pham, S. H., Nguyen, T. T. N., Nguyen, T. T. T., & Bui, T. B. (2025). Extraction process optimization for maximizing total polyphenol content in cashew nut testa (Anacardium occidentale L.) extract. Ho Chi Minh City Open University Journal of Science – Engineering and Technology, 15(1), 34-46. doi:10.46223/HCMCOUJS.tech.en.15.1.3786.2025


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DOI: https://doi.org/10.46223/HCMCOUJS.tech.en.15.1.3786.2025
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