Mycelial cultivation of Phlebopus spongiosus, an edible ectomycorrhizal mushroom in Southern Vietnam

Authors

  • Le Thi Thuy Nhi
    Biotechnology Center of Ho Chi Minh City, VN
  • Tran Chi Hieu
    Biotechnology Center of Ho Chi Minh City, VN
  • Phan My Hanh
    Biotechnology Center of Ho Chi Minh City, VN
  • Bui Van Tan
    Biotechnology Center of Ho Chi Minh City, VN
  • Pham Nguyen Duc Hoang
    Biotechnology Center of Ho Chi Minh City, VN
  • Ho Bao Thuy Quyen
    Ho Chi Minh City Open University, VN

Keywords:

Phlebopus spongiosus; mycelialcultivation; edible ectomycorrhizal mushroom (EEMM); chlamydospore.

Abstract

Phlebopus spongiosus is an endemic edible ectomycorrhizal mushroom (EEMM) in Southern Vietnam. Strains of Phlebopus spongiosus – collected from Pomelo orchards, Ben Tre Province, Vietnam – were cultured on different media, temperatures, pH and carbon/nitrogen sources to define mycelial growth conditions for spawn mushroom production. Phlebopus spongiosus BC-F0075 grew the fastest on the modified MS medium. The optimum temperature for mycelial growth was 30°C and the optimum pH was 4 – 5. BC-F0075 grew well on the MS media containing saccharose, glucose, fructose and maltose, but did not with lactose. Additionally, NH4H2PO4 was reported to be the best nitrogen source to the growth of BC-F0075 mycelia, whereas urea ((NH2)2CO) was not utilized as a nitrogen source.

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References

Giomaro, G., Sisti, D. and Zambonelli, A. (2005). Cultivation of edible ectomycorrhizal fungi by in vitro mycorrhizal synthesis. In: Declerck, S., Strullu, D. G. and Fortin, J.A. (Eds.), In vitro cultivation of mycorrhizas. Soil Biology, 4, 253-270. Springer -Verlag Berlin Heidelberg.

Hall, I. R., Wang, Y. and Amicucci A. (2003). Cultivation of edible ectomycorrhizal mushrooms. Trends in Biotechnology, 21, 433-438.

Izumitsu, K., Hatoh, K., Sumita, T., Kitade, Y., Morita, A., Gafur, A., Ohta, A., Kawai, M., Yamanaka, T., Neda, H., Ota, Y. and Tanaka, C. (2012). Rapid and simple preparation of mushroom DNA directly from colonies and fruiting bodies for PCR. Mycoscience, 53, 396-401.

Ji, K., Cao, Y., Zhang, C., He, M., Liu, J., Wang, W., Wang, Y. (2011). Cultivation of Phlebopus portentosus in southern China. Mycological Progress, 10, 293-300.

Kumla, J., Danell, E., Bussaban, B. and Lumyong, S. (2011). Suitable growth conditions and nutrition factors on in vitro culture of Phlebopus portentosus (Boletales). Chiang Mai Journal Science, 38, 156-159.

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Received: 04-06-2020
Accepted: 04-06-2020
Published: 09-08-2017

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How to Cite

Nhi, L. T. T., Hieu, T. C., Hanh, P. M., Tan, B. V., Hoang, P. N. D., & Quyen, H. B. T. (2017). Mycelial cultivation of Phlebopus spongiosus, an edible ectomycorrhizal mushroom in Southern Vietnam. HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY, 7(1), 14–21. Retrieved from https://journalofscience.ou.edu.vn/index.php/tech-en/article/view/364

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Copyright (c) 2017 Le Thi Thuy Nhi; Tran Chi Hieu; Phan My Hanh; Bui Van Tan; Pham Nguyen Duc Hoang; Ho Bao Thuy Quyen

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.