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7 (1) 2017

Mycelial cultivation of Phlebopus spongiosus, an edible ectomycorrhizal mushroom in Southern Vietnam


Author - Affiliation:
Le Thi Thuy Nhi - Biotechnology Center of Ho Chi Minh City , Vietnam
Tran Chi Hieu - Biotechnology Center of Ho Chi Minh City , Vietnam
Phan My Hanh - Biotechnology Center of Ho Chi Minh City , Vietnam
Bui Van Tan - Biotechnology Center of Ho Chi Minh City , Vietnam
Pham Nguyen Duc Hoang - Biotechnology Center of Ho Chi Minh City , Vietnam
Ho Bao Thuy Quyen - Ho Chi Minh City Open University , Vietnam
Corresponding author: Le Thi Thuy Nhi - thuynhi.sh@gmail.com

Abstract
Phlebopus spongiosus is an endemic edible ectomycorrhizal mushroom (EEMM) in Southern Vietnam. Strains of Phlebopus spongiosus – collected from Pomelo orchards, Ben Tre Province, Vietnam – were cultured on different media, temperatures, pH and carbon/nitrogen sources to define mycelial growth conditions for spawn mushroom production. Phlebopus spongiosus BC-F0075 grew the fastest on the modified MS medium. The optimum
temperature for mycelial growth was 30°C and the optimum pH was 4 – 5. BC-F0075 grew well on the MS media containing saccharose, glucose, fructose and maltose, but did not with lactose. Additionally, NH4H2PO4 was reported to be the best nitrogen source to the growth of BC-F0075 mycelia, whereas urea ((NH2)2CO) was not utilized as a nitrogen source.

Keywords
Phlebopus spongiosus; mycelialcultivation;edible ectomycorrhizal mushroom (EEMM); chlamydospore.

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