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10 (1) 2020

Studies on antimicrobial activities of endophytic bacteria isolated from Neem tree (Azadirachta indica)


Author - Affiliation:
Duong Nhat Linh - Ho Chi MinhCity Open University , Vietnam
Bui Van Thien - Ho Chi Minh City Open University , Vietnam
Tran Thi Ai Ni - Midoli Co., ltd , Vietnam
Nguyen Thanh Duy - University of San Francisco, USA , Vietnam
Nguyen Van Minh - Ho Chi Minh City Open University , Vietnam
Tran Cat Dong - University of Medicine and Pharmacy at Ho Chi Minh City , Vietnam
Corresponding author: Duong Nhat Linh - linh.dn@ou.edu.vn

Abstract
The objective of this study is to isolate endophytic bacteria from Azadirachta indica (neem). We isolated 7 strains of endogenous bacteria from the neem tree. By investing the anti-fungal and antibacterial activities of endophytic bacteria in A. indica by well diffusion agar method, we noticed there was KT2 strain which resisted to Salmonella typhi and Staphylococcus aureus (10.67 ± 0.33 mm and 9.67 ± 0.33 mm), KT3 strain showed the inhibitory activity to three human pathogenic fungal (Candida albicans, Trichophyton mentagrophytes, Trichophyton rubrum), two strains (KT1, KT2) which resisted to C. albicans and T. rubrum. According to Cowan and Steel's manual, the result of biochemical identification showed that the KT2 strain was the Bacillus subtilis species.

Keywords
Azadirachta indica A. Juss; bacillus subtilis; Candida albicans; endophytic bacteria; trichophyton rubrum

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Cite this paper as:

Duong, N. L., Bui, V. T., Tran, T. A. N., Nguyen, T. D., Nguyen, V., M., & Tran, C. D. (2020). Studies on antimicrobial activities of endophytic bacteria isolated from Neem tree (Azadirachta indica). Ho Chi Minh City Open University Journal of Science – Engineering and Technology, 10(1), 57-64.


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DOI: https://doi.org/10.46223/HCMCOUJS.tech.en.10.1.362.2020
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