Studies on antimicrobial activities of endophytic bacteria isolated from Neem tree (Azadirachta indica)

Duong Nhat Linh; Bui Van Thien; Tran Thi Ai Ni; Nguyen Thanh Duy; Nguyen Van Minh; Tran Cat Dong

doi:10.46223/HCMCOUJS.tech.en.10.1.362.2020

Authors

Duong Nhat Linh linh.dn@ou.edu.vn
Ho Chi MinhCity Open University
Bui Van Thien
Ho Chi Minh City Open University
Tran Thi Ai Ni
Midoli Co., ltd
Nguyen Thanh Duy
University of San Francisco, USA
Nguyen Van Minh
Ho Chi Minh City Open University
Tran Cat Dong
University of Medicine and Pharmacy at Ho Chi Minh City

DOI:

https://doi.org/10.46223/HCMCOUJS.tech.en.10.1.362.2020

Keywords:

Azadirachta indica A. Juss, bacillus subtilis, Candida albicans, endophytic bacteria, trichophyton rubrum

Abstract

The objective of this study is to isolate endophytic bacteria from Azadirachta indica (neem). We isolated 7 strains of endogenous bacteria from the neem tree. By investing the anti-fungal and antibacterial activities of endophytic bacteria in A. indica by well diffusion agar method, we noticed there was KT2 strain which resisted to Salmonella typhi and Staphylococcus aureus (10.67 ± 0.33 mm and 9.67 ± 0.33 mm), KT3 strain showed the inhibitory activity to three human pathogenic fungal (Candida albicans, Trichophyton mentagrophytes, Trichophyton rubrum), two strains (KT1, KT2) which resisted to C. albicans and T. rubrum. According to Cowan and Steel's manual, the result of biochemical identification showed that the KT2 strain was the Bacillus subtilis species.

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