Evaluating of chlorpyrifos-degrading by bacterial strains in mineral salt minimum and in the soil

Abstract

Four bacterial strains degraded chlorpyrifos, isolated from agricultural soil, were used as a source of bacteria to investigate their ability to decompose chlorpyrifos in mineral salt minimum and the soil. Barrientosimonas humi C4.3 was investigated for the decomposition of chlorpyrifos in this strain on different days (10, 20 and 30 days of culture) as supplemented and not supplemented TSB. At the same time, another experiment was carried out to evaluate the chlorpyrifos etherification of B. humi C4.3 and the four strains of Achromobacter xylosoxidans C3.1, B. humi C4.3, Microbacterium sp. C8.9, Staphylococcus pasteuri C9.2 in a soil environment. The experiment was carried out including 3 treatments, each treatment was repeated 3, two soil types (sterile soil and non-sterile soil) and bacteria (single bacteria and four bacterial species). The results showed that, in the same culture period of 30 days incubation, biodegradable chlorpyrifos of B. humi C4.3 in the mineral salt medium was more effective (63.07% biodegradable chlorpyrifos) than when grown in soil (21.4% biodegradable chlorpyrifos). Also, biodegradable chlorpyrifos of B. humi C4.3 that was cultured in sterile soil was higher than in non-sterile soil.