--

10 (1) 2020

A comparison between Hydrochloric acid and Trifluoroacetic acid in hydrolysis method of exopolysaccharide from Ophiocordyceps sinensis in Monosaccharide composition analysis by GC-FID


Author - Affiliation:
Le Thi Thuy Hang - Institute of Tropical Biology, Vietnam Academy of Science and Technology; Graduate University of Science and Technology, Vietnam Academy of Science and Technology; Faculty of Food Technology, Ho Chi Minh City University of Food Industry , Vietnam
Ha Bao Chau - Faculty of Biology & Biotechnology, VNUHCM-University of Science , Vietnam
Tran Van Hai Nam - Faculty of Biology & Biotechnology, VNUHCM-University of Science , Vietnam
Pham Minh Thong - Faculty of Biology & Biotechnology, VNUHCM-University of Science , Vietnam
Dang Hoang Phu - Department of Organic Chemistry, Faculty of Chemistry, VNUHCM-University of Science , Vietnam
Dinh Minh Hiep - Ho Chi Minh City Agricultural Hi-Tech Park, Ho Chi Minh City , Vietnam
Nguyen Tien Thang - Institute of Tropical Biology, Vietnam Academy of Science and Technology; Graduate University of Science and Technology, Vietnam Academy of Science and Technology , Vietnam
Corresponding author: Le Thi Thuy Hang - hangltt@cntp.edu.vn

Abstract
The monosaccharide composition is one of the crucial factors affecting the bioactivity of exopolysaccharide (EPS) in Cordyceps species. Therefore, many scientists have studied, analyzed monosaccharide composition and structure of EPS from Cordyceps species, especially Ophiocordyceps sinensis (O. sinensis). This study aimed to compare hydrochloric acid (HCl) with trifluoroacetic acid (TFA) in the EPS hydrolysis process in monosaccharide composition analysis by Gas Chromatography with Flame-Ionization Detection (GC-FID). The hydrolysis is a crucial step in forming the acetyl derivative, which helps the GC-FID technique to have good results in monosaccharide composition analysis. The results showed that hydrolysis with HCl gave a higher hydrolysis efficiency and was more suitable than hydrolysis by TFA in pretreatment to EPS for GC-FID. Hydrolysis results were analyzed through thin-layer chromatography and high-performance liquid chromatography (HPLC), then Acetyl derivatives were produced and finally analyzed by GC-FID to determine the monosaccharide composition of EPS. For EPS hydrolyzed by HCl, the analytical results presented that this sample had 6 kinds of monosaccharides, including rhamnose, arabinose, xylose, mannose, glucose, and galactose; the most monosaccharide was glucose. The EPS hydrolyzed by TFA only detected three kinds of monosaccharides, including mannose, arabinose, and galactose, mainly mannose. The study has set a foundation for further analysis of monosaccharide composition and structure of EPS from O. sinensis.

Keywords
EPS hydrolysis; GC-FID; HPLC; monosaccharide; O. sinensis

Full Text:
PDF

Cite this paper as:

Le, T. T. H., Ha, B. C., Tran, V. H. N., Pham, M. T., Dang, H. P., Dinh, M. H., & Nguyen, T. T. (2020). A comparison between hydrochloric acid and trifluoroacetic acid in hydrolysis method of exopolysaccharide from ophiocordyceps sinensis in monosaccharide composition analysis by GC-FID. Ho Chi Minh City Open University Journal of Science – Engineering and Technology, 10(1), 21-27.


References

Cha, S. H., Lim, J. S., Yoon, C. S., Koh, J. H., Chang, H. I., & Kim, S. W. (2007). Production of mycelia and exo-biopolymer from molasses by Cordyceps sinensis 16 in submerged culture. Bioresource Technology, 98(1), 165-168.


Hon, D. N. S. (2001). Cellulose: Chemistry and technology. In K. H. J. Buschow, R. W. Cahn, M. C. Flemings, B. Ilschner, E. J. Kramer, S. Mahajan & P. Veyssière (Eds.), Encyclopedia of materials: Science and technology (pp. 1039-1045). Oxford, UK: Elsevier.


Lo, H. C., Hsieh, C., Lin, F. Y., & Hsu, T. H. (2013). A systematic review of the mysterious caterpillar fungus ophiocordyceps sinensis in Dong-ChongXiaCao (Dong Chong Xia Cao) and related bioactive ingredients. Journal of Traditional and Complementary Medicine, 3(1), 16-32.


Merkle, R. K., & Poppe, I. (1994). Carbohydrate composition analysis of glycoconjugates by gas-liquid chromatography/mass spectrometry. Methods in Enzymology, 230, 1-15.


Nie, S. P., Cui, S. W., & Xie, M. Y. (2018). Cordyceps polysaccharides. In S. Nie, S. W. Cui & M. Xie (Eds.), Bioactive polysaccharides (pp. 143-204). London, UK: Academic Press.


Selvendran, R. R., March, J. F., & Ring, S. G. (1979). Determination of aldoses and uronic acid content of vegetable fiber. Analytical Biochemistry, 96(2), 282-292.


Soltani, M., Kamyab, H., & El-Enshasy, H. A. (2013). Molecular weight (Mw) and monosaccharide composition (MC): Two major factors affecting the therapeutic action of polysaccharides extracted from cordyceps sinensis. Journal of Pure and Applied Microbiology, 7(3), 1601-1613.


Vo, T. P. T. (2018). Study on methods of obtaining exopolysaccharide fractions from Ophiocordyceps sinensis culture (Master’s thesis). VNUHCM-University of Science, Ho Chi Minh city, Vietnam.


Yan, J. K., Wang, W. Q., & Wu, J. Y. (2014). Recent advances in Cordyceps sinensis polysaccharide: Mycelial fermentation, isolation, structure, and bioactivities: A review. Journal of Functional Foods, 6, 33-47.


Yang, B., Yu, G., Zhao, X., Jiao, G., Ren, S., & Chai, W. (2009). Mechanism of mild acid hydrolysis of galactan polysaccharides with highly ordered disaccharide repeats leading to a complete series of exclusively odd‐numbered oligosaccharides. The FEBS Journal, 276(7), 2125-2137.


Yu, G., Guan, H., Ioanoviciu, A. S., Sikkander, S. A., Thanawiroon, C., & Tobacman, J. K. (2002). Structural studies on k-carrageenan derived oligosaccharides. Carbohydrate Research, 337, 433-440.


Yuan, Y., Wang, Y. B., Jiang, Y., Prasad, K. N., Yang, J., Qu, H., … Yang, B. (2016). Structure identification of a polysaccharide purified from Lycium barbarium fruit. International Journal of Biological Macromolecules, 82, 696-701.


Zhang, W., Yang, J., Chen, J., Hou, Y., & Han, X. (2005). Immunomodulatory and antitumour effects of an exopolysaccharide fraction from cultivated Cordyceps sinensis (Chinese caterpillar fungus) on tumour‐bearing mice. Biotechnology and Applied Biochemistry, 42(1), 9-15.



DOI: https://doi.org/10.46223/HCMCOUJS.tech.en.10.1.357.2020
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.