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10 (1) 2020

Isolation and selection of Bacillus strains with high potential probiotic that used in catfish farming (Pangasianodon hypophthalmus)


Author - Affiliation:
Nguyen Van Minh - Faculty of Biotechnology, Ho Chi Minh City Open University , Vietnam
Nguyen Hoang Tuan Duy - Faculty of Biotechnology, Ho Chi Minh City Open University , Vietnam
Do Phuong Quynh - Faculty of Biotechnology, Ho Chi Minh City Open University , Vietnam
Tran Thi A Ni - MIDOLI Co., ltd , Vietnam
Tran Kien Duc - Ho Chi Minh City University of Science , Vietnam
Duong Nhat Linh - Faculty of Biotechnology, Ho Chi Minh City Open University , Vietnam
Corresponding author: Nguyen Van Minh - minh.nv@ou.edu.vn

Abstract
In this study, we isolated 28 strains of Bacillus spp. from water samples, catfish pond mud samples and earthworm manure (Perionyx excavates). By the cross-streak agar methods, 22 Bacillus strains showed the inhibition ability to Edwardsiella ictaluri, which caused Bacillary Necrosis Pangasius (BNP) in catfish (Pangasianodon hypophthalmus). Both Bacillus sp. Q16 and Q111 strains showed the highest inhibition to E. ictaluri by the double-layer agar methods. Finally, two Bacillus strains (Q16, Q111) were selected as a source of potential probiotic because of the ability of extracellular enzyme secretion (protease, amylase, cellulose) strong growth at 0,1-1% salt concentrations, survival within the pH range 6-8, resistance to low pH and low bile salts, inability to produce haemolysin enzyme, sensitivity to eight antibiotics in the three impacting groups (inhibition of wall synthesis, inhibition mechanism of protein synthesis, inhibition of nucleic acid synthesis). Two Bacillus strains (Q16, Q111) were identified that they belong to Bacillus subtilis by biochemical method and 16S rRNA gene sequencing method. This study indicated that two Bacillus strains (Q16, Q111) isolated from catfish pond can be applied as high potential probiotics that used to farm catfish.

Keywords
Bacillus subtilis; probiotic; Edwardsiella ictaluri; Perionyx excavatus;Pangasianodon hypophthalmus

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Nguyen, V. M., Nguyen, H. T. D., Do, P. Q., Tran, T. A. N., Tran, K. D., & Duong, N. L. (2020). Isolation and selection of Bacillus strains with high potential probiotic that used in catfish farming (Pangasianodon hypophthalmus). Ho Chi Minh City Open University Journal of Science – Engineering and Technology, 10(1), 3-11.


References

Abriouel, H., Franz, C. M. A. P., Omar, N. B., & Gálvez, A. (2011). Diversity and applications of Bacillus bacteriocins. FEMS Microbiology Reviews, 35(1), 201-232.


Aly, S. M., Ahmed, Y. A. G., Ghareeb, A. A. A., & Mohamed, M. F. (2008). Studies on Bacillus subtilis and Lactobacillus acidophilus, as potential probiotics, on the immune response and resistance of Tilapia nilotica (Oreochromis niloticus) to challenge infections. Fish & Shellfish Immunology, 25(1/2), 128-36. doi:10.1016/j.fsi.2008.03.013


Barrow, G., & Feltham, R. (1993). Cowan and Steel's manual for the identification of medical bacteria. Cambridge, UK: Cambridge University Press.


Cha, J. H., Rahimnejad, S., Yang, S. Y., Kim, K. W., & Lee, K. J. (2013). Evaluations of Bacillus spp. as dietary additives on growth performance, innate immunity and disease resistance of olive flounder (Paralichthys olivaceus) against Streptococcus iniae and as water additives. Aquaculture, 402-403, 50-57. doi:10.1016/j.aquaculture.2013.03.030


Chao, R., Abel, C., Malachi, A. W., Nancy, C., Bui, C. T. D., Joseph, C. N., … Mark, R. L. (2012). Identification of Bacillus strains for biological control of catfish pathogens. PLOS One, 7(9),1-9.


Cowan, S. T., & Steel, K. J. (1993). Cowan and Steel’s manual for identification of medical bacteria (3rd ed.) (pp. 199-241). Cambridge, UK: Cambridge University Press.


Cukrowska, B., Motyl, I., Kozáková, H., Schwarzer, M., Górecki, R. K., Klewicka, E., … Libudzisz, Z. (2009). Probiotic Lactobacillus Strains: In vitro and in vivo Studies, Folia Microbiologica, 54(6), 533-537.


Dang, O. T. H., & Nguyen, P. T. (2012). Trial of Edwardsiella ictaluri on Pangasius hypophthalmus by Erythromycin Thiocynate antibiotic. Can Tho University Journal of Science, 2012(22c), 146-154.


Hankin, L., & Anagnostakis, S. L. (1975). The use of solid media for detection of enzyme production by fungi. Cryptogamie, Mycologie, 67(3), 597- 607.


Ho, T. T. T., Nguyen, H. T., Nguyen, N. T., Ong, M. Q., Korntip, K., Sasimamas, U., & Nontawith, A. (2017). Identification and characterization of potential probiotic Bacillus spp. for application in striped catfish (Pangasianodon hypophthalmus [Sauvage, 1878]). Journal of Fisheries and Environment, 41(2), 20-32.


Holt, J. G., Krieg, N. R., Sneath, P. H. A., Staley, J. T., & Williams S. T. (1994). Bergey's manual of determinative bacteriology (9th ed.). Philadelphia, PA: Lippincott Williams & Wilkins.


Huynh, A. H., Le, H. D., & Cutting, S. M. (2005). The use of bacterial spore formers as probiotics. FEMS Microbiol Reviews, 29(4), 813-835. doi:10.1016/j.femsre.2004.12.001


Hyronimus, B., Marrec, C. L., Sassi, A. H., & Deschamps, A. (2000). Acid and bile tolerance of spore-forming lactic acid bacteria. International Journal of Food Microbiology, 61(2/3), 193-197. doi:10.1016/s0168-1605(00)00366-4


Jock, S., Völksch, B., Mansvelt, L., & Geider, K., (2002). Characterization of Bacillus strains from apple and pear trees in South Africa antagonistic to Erwinia amylovora. FEMS Microbiology Letters, 211(2), 247-252. doi:10.1111/j.1574-6968.2002.tb11232.x


Joo, M., Hur, S., Han, Y., & Kim, J. (2007). Isolation, indentification and characterization of Bacillus strains from the traditional Korean soybean-fermented food, Chungkookjang. Journal of Applied Biological Chemistry, 50(4), 202-210.


Kwon, G. H., Lee, H. A., Park, J. Y., Kim, J. S., Park, C. S., Kwon, D. Y., … Kim, J. H. (2009). Development of a RAPD-PCR method for identification of Bacillus species isolated from Cheonggukjang. International Journal of Food Microbiology, 129(3), 282-287. doi:10.1016/j.ijfoodmicro.2008.12.013


Lemos, M. L., Toranzo, A. E., & Barja, J. L. (1985). Antibiotic activity of epiphytic bacteria isolated from intertidal seaweeds. Microbial Ecology, 11(2), 149-163. doi:10.1007/BF02010487


Ma, C. W., Cho, Y. S., & Oh, K. H. (2009). Removal of pathogenic bacteria and nitrogens by Lactobacillus spp. JK‐8 and JK‐11. Aquaculture, 287(3/4), 266-270. doi:10.1016/j.aquaculture.2008.10.061


Montville, T. J. (1983). Dual-substrate plate diffusion assay for proteases. Applied and Environmental Microbiology, 45(1), 200-204.


Moriarty, D. J. W. (1997). The role of microorganisms in aquaculture ponds. Aquaculture, 151(1/4), 333-349.


Moriarty, D. J. W. (2006). Safety of aquaculture probiotics. Global Aquaculture Advocate, 86-87.


Nguyen, M. V., Nguyen, D. H. T., Do, Q. P., Vo, N. N. Y., Duong, L. N., Nguyen, T. T. N., & Le, P. H. (2013). Biocontrol of pathogenic Edwardsiella ictaluri by Bacillus spp. isolated from catfish pond. Journal of Science and Technology, 51(5C), 508-512.


Samanta, R., Pal, D., & Sem, S. P. (1989). Production of hydrolases by N2 fixing microorganisms. Biochemie and Physiologie der pflanzen, 185(1/2), 75-81.


Sánchez-Ortiz, A. C., Luna-González, A., Campa-Córdova, A. I., Escamilla-Montes, R., Flores-Miranda, M. C., & Mazon-Suastegui, J. M. (2015), Isolation and characterization of potential probiotic bacteria from pustulosebark (Anadara tuberculosa) suitable for shrimp farming. Latin American Journal of Aquatic Research, 43(1), 123-136.


Schwalbe, R., Steele, M. L., & Goodwin, A. C. (2007). Antimicrobial susceptibility testing protocols. Boca Raton, FL: CRC Press.


Shafiqur, R., Shakila, N. K., Niamul, N. M., & Manjurul, K. M. (2009). Application of probiotic bacteria: A novel approach towards ensuring food safety in shrimp aquaculture. Journal of Bangladesh Academy of Sciences, 33(1), 139-144.


Sudha, M. R., Chauhan, P., Dixit, K., Babu, S., & Jamil, K. (2010). Molecular typing and probiotic attributes of a new strain of Bacillus coagulans - Unique IS-2: A potential biotherapeutic agent. Genetic Engineering and Biotechnology Journal GEBJ, 7, 1-20.


Vo, M. S., Van, T. T., & Nguyen, T. N. T. (2013). Dietary administration of the probiotic enhanced innate immune responses and disease resistance of the striped catfish against Edwardsiella ictaluri. Journal of Agriculture and Rural Development, 14, 81-89.



DOI: https://doi.org/10.46223/HCMCOUJS.tech.en.10.1.355.2020
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