--

9 (1) 2019

Applied bioinformatics tools for analysis of Microrna-214 expression and prediction of its potential targets genes in Nasopharyngeal carcinoma


Author - Affiliation:
Nguyen Hoang Danh - Ho Chi Minh City Open University , Vietnam
Thieu Hong Hue - University of Science, Vietnam National University Ho Chi Minh City , Vietnam
Quang Trong Minh - Ho Chi Minh City Open University , Vietnam
K' Trong Nghia - Ho Chi Minh City Open University , Vietnam
Nguyen Thanh Tung - Ho Chi Minh City Open University , Vietnam
Phan Dang Hoang Nam - Ho Chi Minh City Open University , Vietnam
Tran Hoang Bao Tran - Ho Chi Minh City Open University , Vietnam
Lao Duc Thuan - Ho Chi Minh City Open University; University of Science, Vietnam National University Ho Chi Minh City , Vietnam
Corresponding author: Lao Duc Thuan - thuan.ld@ou.edu.vn

Abstract
miRNA (microRNA) are short RNA molecules in length from 20 to 24 nucleotides that have been shown to play an important role in regulating gene expression in many different types of human cancer. Meanwhile, miRNA-214 is one of the known miRNAs involved in the formation of nasopharyngeal carcinoma (NPC) through overexpression that promotes proliferation and development of cancer cells. However, in Vietnam, the study of miR-214 related to NPC has not been conducted yet. With the aims to develop the further studies of miR-214 on NPC in Vietnamese patients, in this initial study, we conducted the analysis of miR-214 expression in previous publications, as well as the prediction of miR-214 potential target genes, which involved in many cellular pathways. Here we applied bioinformatics tools to predict miRNAs and their targets, and discuss the role of miR-214 in the context of human cancers. As the results, miR-214 acted as the oncogenic roles in NPC, relevanted to many pathways, such as cell proliferation, apoptosis, metastasis and invasion through the its target genes LTF, Bim, Bax, LINC0086, etc. In conclusion, the use of computional approaches facilitate the further experimental validation of miRNAs in general, particularly miR-214, in Vietnamese NPC patients.

Keywords
microRNA;miRNA-214; Nasopharyngeal carcinoma

Full Text:
PDF

References

Deng, M., Ye, Q., Qin, Z., Zheng, Y., He, W., Tang, H., … Li, G. (2013). miR-214 promotes tumorigenesis by targeting lactotransferrin in nasopharyngeal carcinoma. Tumor Biology, 34(3), 1793-1800.


Derfoul, A., Juan, A. H., Difilippantonio, M. J., Palanisamy, N., Ried, T., & Sartorelli, V. (2011). Decreased microRNA-214 levels in breast cancer cells coincides with increased cell proliferation, invasion and accumulation of the Polycomb Ezh2 methyltransferase. Carcinogenesis, 32(11), 1607-1614.


Filipowicz, W., Bhattacharyya, S. N., & Sonenberg, N. (2008). Mechanisms of posttranscriptional regulation by microRNAs: Are the answers in sight? Nature Reviews Genetics, 9(2), 102-114.


Guo, J., Ma, J., Zhao, G., Li, G., Fu, Y., Luo, Y., & Gui, R. (2017). Long noncoding RNA LINC0086 functions as a tumor suppressor in nasopharyngeal carcinoma by targeting miR-214. Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics, 25(7), 1189-1197.


He, J., Tang, Y., & Tian, Y. (2015). MicroRNA 214 promotes proliferation and inhibits apoptosis via targeting Bax in nasopharyngeal carcinoma cells. Molecular Medicine Reports, 12(4), 6286-6292.


Khoo, A. S. B., & Pua, K. C. (2013). Diagnosis and clinical evaluation of nasopharyngeal carcinoma. In Nasopharyngeal Carcinoma (pp. 1-9). New York, NY: Springer.


Lan, H., Lu, H., Wang, X., & Jin, H. (2015). MicroRNAs as potential biomarkers in cancer: Opportunities and challenges. BioMed Research International, 2015, 1-17.


Lee, K. T. W., Tan, J. K., Lam, A. K. Y., & Gan, S. Y. (2016). MicroRNAs serving as potential biomarkers and therapeutic targets in nasopharyngeal carcinoma: A critical review. Critical Reviews in Oncology/Hematology, 103, 1-9.


Lewis, B. P., Burge, C. B., & Bartel, D. P. (2005). Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell, 120(1), 15-20.


Li, S., Hang, L., Ma, Y., & Wu, C. (2016). Distinctive micro RNA expression in early stage nasopharyngeal carcinoma patients. Journal of Cellular and Molecular Medicine, 20(12), 2259-2268.


Lu, J., Xu, X., Liu, X., Peng, Y., Zhang, B., Wang, L., ... Li, X. (2014). Predictive value of miR-9 as a potential biomarker for nasopharyngeal carcinoma metastasis. British Journal of Cancer, 110(2), 392-398.


Narducci, M., Arcelli, D., Picchio, M., Lazzeri, C., Pagani, E., Sampogna, F., ... Facchiano, A. (2011). MicroRNA profiling reveals that miR-21, miR486 and miR-214 are upregulated and involved in cell survival in Sezary syndrome. Cell Death & Disease, 2(4), 1-8.


Penna, E., Orso, F., Cimino, D., Tenaglia, E., Lembo, A., Quaglino, E., . . . De Pittà, C. (2011). MicroRNA‐214 contributes to melanoma tumour progression through suppression of TFAP2C. The EMBO Journal, 30(10), 1990-2007.


Plieskatt, J. L., Rinaldi, G., Feng, Y., Levine, P. H., Easley, S., Martinez, E., … Mulvenna, J. P. (2014). Methods and matrices: Approaches to identifying miRNAs for Nasopharyngeal carcinoma. Journal of Translational Medicine, 12(1), 1-20.


Shih, T. C., Tien, Y. J., Wen, C. J., Yeh, T. S., Yu, M. C., Huang, C. H., … Hsieh, S. Y. (2012). MicroRNA-214 downregulation contributes to tumor angiogenesis by inducing secretion of the hepatoma-derived growth factor in human hepatoma. Journal of Hepatology, 57(3), 584-591.


Ueda, T., Volinia, S., Okumura, H., Shimizu, M., Taccioli, C., Rossi, S., … Yasui, W. (2010). Relation between microRNA expression and progression and prognosis of gastric cancer: A microRNA expression analysis. The Lancet Oncology, 11(2), 136-146.


Yang, H., Kong, W., He, L., Zhao, J.-J., O'Donnell, J. D., Wang, J., … Nicosia, S. V. (2008). MicroRNA expression profiling in human ovarian cancer: miR-214 induces cell survival and cisplatin resistance by targeting PTEN. Cancer Research, 68(2), 425-433.


Yang, Z., Chen, S., Luan, X., Li, Y., Liu, M., Li, X., ... Tang, H. (2009). MicroRNA‐214 is aberrantly expressed in cervical cancers and inhibits the growth of HeLa cells. IUBMB Life, 61(11), 1075-1082.


Yin, G., Chen, R., Alvero, A. B., Fu, H.-H., Holmberg, J., Glackin, C., … Mor, G. (2010). TWISTing stemness, inflammation and proliferation of epithelial ovarian cancer cells through MIR199A2/214. Oncogene, 29(24), 3545-3553.


Yuan, H. L., Wang, T., & Zhang, K. H. (2018). MicroRNAs as potential biomarkers for diagnosis, therapy and prognosis of gastric cancer. OncoTargets and Therapy, 11, 3891-3900.


Zhang, X. J., Ye, H., Zeng, C. W., He, B., Zhang, H., & Chen, Y. Q. (2010). Dysregulation of miR-15a and miR-214 in human pancreatic cancer. Journal of Hematology & Oncology, 3(1), 1-9.


Zhang, Z. C., Li, Y. Y., Wang, H. Y., Fu, S., Wang, X. P., Zeng, M. S., … Shao, J. Y. (2014). Knockdown of miR-214 promotes apoptosis and inhibits cell proliferation in nasopharyngeal carcinoma. PloS One, 9(1), e86149.



DOI: https://doi.org/10.46223/HCMCOUJS.tech.en.9.1.347.2019
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.