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8 (2) 2018

Screening of endophytes from rubber trees (hevea brasiliensis) for biological control of Corticium salmonicolor


Author - Affiliation:
Nguyen Van Minh - Ho Chi Minh City Open University , Vietnam
Mai Huu Phuc - Ho Chi Minh City Open University , Vietnam
Duong Nhat Linh - Ho Chi Minh City Open University , Vietnam
Tran Thi A Ni - Midoli co. ltd , Vietnam
Tran Kien Duc - University of Science, Viet Nam National University Ho Chi Minh City , Vietnam
Nguyen Anh Nghia - Rubber research institute of Viet Nam , Vietnam
Corresponding author: Nguyen Van Minh - minh.nv@ou.edu.vn

Abstract
28 leaves and living-tissue samples of rubber tree (Hevea brasiliensis) were collected from Ho Chi Minh City, Binh Phuoc province and Binh Duong province (Viet Nam). We isolated and screened endophytes that have potential application as agents for biocontrol of Corticium salmonicolor, the agent of Pink Disease in rubber trees. As a result, 21 strains of endophytic bacteria and 14 strains of endophytic fungi were isolated. Antagonistic activity of the endophytes towards C. salmonicolor was checked by using a dual culture. Testing results showed that: T9, T15 and T16 strains have inhibited C. salmonicolor. T9 and T16 strains showed result that 100% of inhibiting C. salmonicolor at the concentration of 1:1. In the test of ability to kill C. salmonicolor, T9 and T16 strains showed that they could kill C. salmonicolor
after 3 sprays of bacterial filtrate. T9 and T6 strains, which were identified by biochemical methods, have similar characteristics to Bacillus thuringiensis.

Keywords
antagonistic activity test; biocontrol;corticium salmonicolor;endophtytes;heveabrasiliensis

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DOI: https://doi.org/10.46223/HCMCOUJS.tech.en.8.2.345.2018
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