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8 (2) 2018

Construction of a dataset for the gene nrLSU to support the identification of cantharellus at Langbian Mountain, Lam Dong


Author - Affiliation:
Trinh Van Hanh - University of Science, VNU-HCM , Vietnam
Vu Tien Luyen - University of Science, VNU-HCM , Vietnam
Pham Huu Hung - Tay Nguyen Institute of Scientific Research, Vietnam Academy of Science and Technology , Vietnam
Truong Binh Nguyen - Da Lat University , Vietnam
Tran Duc Vinh - Ho Chi Minh City Open University , Vietnam
Lao Duc Thuan - Ho Chi Minh City Open University , Vietnam
Le Huyen Ai Thuy - Ho Chi Minh City Open University , Vietnam
Corresponding author: Le Huyen Ai Thuy - thuy.lha@ou.edu.vn

Abstract
Fungal species of Cantharellales order are found worldwide including Europe, Africa, Asia, and North America. This clade includes two smaller, separate, but closely related genera Cantharellus and Craterellus. Cantharellus was firstly identified in the middle of the 17th century. Besides traditional classification based on morphology, molecular phylogeny of the nuclear ribosomal large subunit gene (nrLSU) can be an effective tool to
provide more information during species identification for this group. In this current report, a dataset of the nrLSU gene including 60 sequences of Cantharellus species and 1 sequence belonging to Craterellus (outgroup), was obtained from Genbank. Phylogenetic analyses by MEGA 6.0 showed a monophyletic relationship between the six well-established clades, namely Cantharellus (Clade 1), Rubrinus (Clade 2), Cinnabarinus (Clade 3), Parvocantharellus (Clade 4), Pseudocantharellus (Clade 5) and subgenus Afrocantharellus (Clade 6) similar to that of morphological classification. Therefore, this dataset is efficient to support the identification of the fungal samples, collected at the Langbian Mountain, Lam Dong.

Keywords
Cantharellus;Large Subunit (LSU) of rDNA; the phylogenetic tree

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DOI: https://doi.org/10.46223/HCMCOUJS.tech.en.8.2.342.2018
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