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8 (1) 2018

Research antioxidant activity of chitooligosacchride by UV-VIS absorption spectrocopy


Author - Affiliation:
Bui Van Hoai - University of Science, Viet Nam National University Ho Chi Minh City , Vietnam
Ngo Dai Nghiep - University of Science, Viet Nam National University Ho Chi Minh City , Vietnam
Dao An Quang - Ho Chi Minh City University of Food Industry , Vietnam
Nguyen Thi Nam Phuong - Ho Chi Minh City University of Food Industry , Vietnam
Corresponding author: Bui Van Hoai - hoaibv@cntp.edu.vn

Abstract
Chitosan with 80% degree of deacetylation was hydrolyzed by cellulase of Trichoderma viride to prepare chitooligosaccharides (COSs) by the fractionation of the COSs with ultrafiltration membrane. The antioxidant
activities of the COSs were clarified in this study by reducing power and free radical scavenging ability assay by UV-VIS absorption spectrum. The results show that the COS 1 (10,000-5,000 Da), COS 2 (5,000-3,000 Da), COS 3 (3,000-1,000 Da) and COS 4 (less than1,000 Da) segments have antioxidant properties.The antioxidant activitives of the COSs increased with the increment of concentration, and they also depended on molecular weight.

Keywords
antioxidant; chitooligosaccharide; DPPH; reducing power; UV-VIS

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DOI: https://doi.org/10.46223/HCMCOUJS.tech.en.8.1.339.2018
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