Tiếng Việt
8 (1) 2018
In silico analysis of hypermethylation in CpGislands of UCHL1 gene’s promoter in nasopharyngeal carcinoma
Corresponding author:Abstract
UCHL1 gene’s promoter in NPC has not been demonstrated yet. In this study, a systematic literature revision was carried out to summarize the current evidences about the frequencies of UCHL-1 gene’s promoter methylation in
NPC for further application in Vietnamese population.
Methods: A systematic literature analysis was conducted based on the comprehensive studies. Moreover, many bioinformatic tools such as Methprimer, TFsearch, IDT OligoAnalyzer 3.1 were used to predict the CpG
islands, transcriptional factors, and to pick up the MSP (Methylation-Specific PCR) primers.
Results: Total of three previous studies were summarized and accessed for eligibility from literature research. As the results, the average weight methylated frequencies were 72.4% and 13.0% for NPC and non-cancerous
samples, respectively. The significant association between UCHL-1 promoter methylation and NPC with the OR of 10.459 (95% CI = 4.915 – 22.254, p < 0.001) and RR of 4.117 (95% CI = 1.958 – 6.645, p < 0.0001) based on the
random effects model, was observed. Moreover, we were successful in predicting the CpG islands as well as identifying transcriptional factor binding sites which served as “hot spot” for ideal primer pick up and located in gene promoter.
Conclusion: The methylation of UCHL-1 gene promoter was significantly associated and contributed to NPC developmentin which it could be further applied in evaluation of UCHL-1 gene promoter status in Vietnamese population.
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References
Ayadi, W., Karray-Hakim, H., Khabir, A., Feki, L., Charfi, S., Boudawara, T., … Hammami, A. (2008). Aberrant methylation of p16, DLEC1, BLU and E-cadherin gene promoters in nasopharyngeal carcinoma biopsies from Tunisian patients. Anticancer Research, 28(4B), 2161-2167.
Caballero, O. L., Resto, V., Patturajan, M., Meerzaman, D., Guo, M. Z., Engles, J., … Jen, J. (2002). Interaction and colocalization of PGP9.5 with JAB1 and p27(Kip1). Oncogene, 21(19), 3003-3010.
Chan, W. H., Chang, K. P., Yang, S. W., Yao, T. C., Ko, T. Y., Lee, Y. S., … Tsai, C. N. (2010). Transcriptional repression of DLEC1 associates with the depth of tumor invasion in oral squamous cell carcinoma. Oral Oncology, 46(12), 874-879.
Da Costa, V. G., Marques-Silva, A. C., & Moreli, M. L. (2015). The Epstein-Barr virus latent membrane protein-1 (LMP1) 30-bp deletion and XhoI-polymorphism in nasopharyngeal carcinoma: a meta-analysis of observational studies. Systematic Reviews, 2015, 13-46.
Dai, W., Zheng, H., Cheung, A. K., & Lung, M. L. (2016). Genetic and epigenetic landscape of nasopharyngeal carcinoma. Chinese Clinical Oncology, 5 (2), 1-13.
Globocan. (2012). Estimated cancer incidence, mortality and prevalence worldwide in 2012. Retrieved January 10, 2018, from http://globocan.iarc.fr/Pages/fact_sheets_population.aspx
Kwong, J., Chow, L. S., Wong, A. Y., Hung, W. K., Chung, G. T., To, K. F., … Lo, K. W. (2007). Epigenetic inactivation of the deleted in lung and esophageal cancer 1 gene in nasopharyngeal carcinoma. Genes Chromosomes Cancer, 46(2), 171-180.
Le, H. A. T., Lao, D. T., & Truong, K. P. (2017). DNA hypermethylation in breast cancer. In P. Pham (Ed.), Breast cancer - From biology to medicine. London, UK: IntechOpen.
Li, L., Tao, Q., Jin, H., van Hasselt, A., Poon, F. F., Wang, X., … Cao, Y. (2010). The tumor suppressor UCHL1 forms a complex with p53/MDM2/ARF to promote p53 signaling and is frequently silenced in nasopharyngeal carcinoma. Clinical Cancer Research, 16(11), 2949-2958.
Loyo, M., Brait, M., Kim, M. S., Ostrow, K. L., Jie, C. C., Chuang, A. Y., … Sidransky, D. (2011). A survey of methylated candidate tumor suppressor genes in nasopharyngeal carcinoma. International Journal of Cancer, 128(6), 1393-1403.
McCabe, D. C., & Caudill, M. A. (2005). DNA methylation, genomic silencing, and links to nutrition and cancer. Nutrition Reviews, 63(6), 183-195.
Pathmanathan, R., Prasad, U., Sadler, R., Flynn, K., & Raab-Traub, N. (1995). Clonal proliferations of cells infected with Epstein-Barr virus in pre-invasive lesions related to nasopharyngeal carcinoma. The New England Journal of Medicine, 333, 693-698.
Shen, H., Sikorska, M., Leblanc, J., Walker, P. R., & Liu, Q. Y. (2006). Oxidative stress regulated expression of ubiquitin Carboxyl-terminal Hydrolase-L1: Role in cell survival. Apoptosis, 11(6), 1049-1059.
Takami, Y., Nakagami, H., Morishita, R., Katsuya, T., Cui, T. X., Ichikawa, T., … Kaneda, Y. (2007). Ubiquitin carboxyl-terminal hydrolase L1, a novel deubiquitinating enzyme in the vasculature, attenuates NF-kappaB activation. Arteriosclerosis, Thrombosis, and Vascular Biology, 27(10), 2184-2190.
Tian, F., Yip, S. P., Kwong, D. L., Lin, Z., Yang, Z., & Wu, V. W. (2013). Promoter hypermethylation of tumor suppressor genes in serum as potential biomarker for the diagnosis of nasopharyngeal carcinoma. Cancer Epidemiol, 37(5), 708-713.
Tokumaru, Y., Yamashita, K., Kim, M. S., Park, H. L., Osada, M., Mori, M., & Sidransky, D. (2008). The role of PGP9.5 as a tumor suppressor gene in human cancer. International Journal of Cancer, 123(4), 753-759.
Tsao, S. W., Yip, Y. L., Tsang, C. M., Pang, P. S., Lau, V. M., Zhang, G., & Lo, K. W. (2014). Etiological factors of nasopharyngeal carcinoma. Oral Oncology, 50(5), 330-338.
Yuanyuan, D., Haiyang, Z., Haiyan, L., Xiaokun, L., & Shulin, Y. (2014). DNA methylation as an early diagnostic marker of cancer (Review). Biomedical Reports, 2(3), 326-330. doi:10.3892/br.2014.237.
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