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14(2)2024

Towards differentiation of Panax vietnamensis and Panax vietnamensis var. fuscidiscus by UPLC-QTOF-MS


Author - Affiliation:
Long Huynh Kim Vu - Ton Duc Thang University, Ho Chi Minh City , Vietnam
Duc Minh Nguyen - Ton Duc Thang University, Ho Chi Minh City , Vietnam
Long Ke Phan - Vietnam National Museum of Nature, Vietnam Academy of Science and Technology, Hanoi , Vietnam
Yen Trang Hai Nguyen - Ton Duc Thang University, Ho Chi Minh City , Vietnam
Huy Truong Nguyen - Ton Duc Thang University, Ho Chi Minh City , Vietnam
Corresponding author: Huy Truong Nguyen - nguyentruonghuy@tdtu.edu.vn
Submitted: 02-11-2023
Accepted: 28-11-2023
Published: 19-04-2024

Abstract
Vietnamese ginseng (Panax vietnamensis Ha et Grusvh., Araliaceae - PV) is a high-value medicinal plant of Vietnam. Recently, a new variety of this plant P. vietnamensis var fuscidiscus (PVF), so-called Lai Chau ginseng, was discovered and circulated on the Vietnam market along with PV. PV and PVF rhizomes and roots are preliminarily reported to possess similar saponin composition. However, PV is much more expensive than PVF due to the higher regard of the consumer, which consequently leads to the inattentive misuse or intentional adulteration of PV by PVF. In this study, a UPLC-ToF-MS method was developed to distinguish PV and PVF based on their saponin profiles. The UPLC qualitative result showed that the saponin constituent of PVF is more complex than that of PV. The significant differences in the chromatogram of PV and PVF extracts are the peaks eluted from 32 - 48 min. Vina-ginsenoside R2 (9), chikusetsusaponin L8 (10), and notoginsenoside R4 (12) are characterized for PVF, while notoginsenoside Fa (13), quinquenoside R1 (16), and pseudoginsenoside Rs1 (19) are specialized for PV. The preliminary quantitative determination result showed that the content of majonoside R2, ginsenoside Rg1, ginsenoside Rb1, and ginsenoside Rd in PV is higher than in PVF. In contrast, the content of majonoside R1 and vina-ginsenoside R2 in PVF is significantly higher than in PV. The developed method could be used as an effective method to distinguish PV and PVF.

Keywords
Lai Chau ginseng; Panax vietnamensis; Panax vietnamensis var. fuscidiscus; UPLC-ToF-MS; Vietnamese ginseng

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Cite this paper as:

Vu, L. H. K., Nguyen, D. M., Phan, L. K., Nguyen, Y. T. H., & Nguyen, H. T. (2024). Towards differentiation of Panax vietnamensis and Panax vietnamensis var. fuscidiscus by UPLC-QTOF-MS. Ho Chi Minh City Open University Journal of Science – Engineering and Technology, 14(2), 19-27. doi:10.46223/HCMCOUJS.tech.en.14.2.3047.2024


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DOI: https://doi.org/10.46223/HCMCOUJS.tech.en.14.2.3047.2024
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