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13 (1) 2023

Disinfection of methicillin-resistant Staphylococcus Aureus on flat surface by 460 nm light and hydrogen peroxide combination


Author - Affiliation:
Truong Nguyen Thuan Thien - International University, Vietnam National University - HCMC , Vietnam
Ngo Nguyen Vu - Nguyen Tat Thanh University, Ho Chi Minh City , Vietnam
Tran Trung Tin - Ho Chi Minh City University of Technology, Vietnam National University - HCMC , Vietnam
Vu Van Van - Nguyen Tat Thanh University, Ho Chi Minh City , Vietnam
Nguyen Thi Thu Hoai - International University, Vietnam National University - HCMC , Vietnam
Vu Minh Thiet - Nguyen Tat Thanh University, Ho Chi Minh City , Vietnam
Corresponding author: Vu Minh Thiet - vmthiet@ntt.edu.vn
Submitted: 16-02-2023
Accepted: 29-03-2023
Published: 05-04-2023

Abstract
Staphyloxanthin (STX) is a carotenoid pigment produced by Staphylococcus aureus to protect the bacteria from oxidation stress. Eliminating Staphyloxanthin from S. aureus cell membrane by inducing the pigment photolysis using 460nm light, then killing weakened bacteria with an H2O2 solution could be a new approach to develop anti - Staphylococcus aureus procedure. A model to prove the feasibility of this combination to kill Methicillin-Resistant Staphylococcus Aureus (MRSA) bacteria on a flat surface was tested. Material and method: Staphyloxanthin pigment extracted from MRSA bacteria was treated with 460nm light at different light intensities to evaluate the photolysis potential of 460nm light. The change in MRSA shape after 460nm light treatment was also investigated by Scanning electron microscopy. Using glass cover-slips as an emulated model for contact surface in public, the combination of different 460nm light intensities and H2O2 0.75% was tested on the surface loaded with MRSA living cells, and the number of MRSA cells survived after treatment was enumerated. Result: Higher intensity and longer light treatment yielded a higher photolysis effect, as 100 and 200 mW/cm2 light intensity could degrade 77.50% to 83.45% of STX pigment after 20 minutes of irradiation. Also, MRSA cells had significant changes as more wrinkles and bumps appeared under high-intensity 460nm light. When tested on the flat surface of the coverslip, the strongest MRSA eradication effect can be observed in the combination of 200 mW/cm2 light treatment with 0.75% H2O2 solution, as 100% MRSA cells were completely killed after 20 minutes of treatment.

Keywords
hydrogen peroxide; methicillin-resistant Staphylococcus aureus; staphyloxanthin; surface disinfection; photolysis; 460nm light

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Cite this paper as:

Truong, T. N. T., Ngo, V. N., Tran, T. T., Vu, V. V., Nguyen, H. T. T., & Vu, T. M. (2023). Disinfection of methicillin-resistant Staphylococcus Aureus on flat surface by 460 nm light and hydrogen peroxide combination. Ho Chi Minh City Open University Journal of Science – Engineering and Technology, 13(1), 10-17. doi:10.46223/HCMCOUJS.tech.en.13.1.2649.2023


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DOI: https://doi.org/10.46223/HCMCOUJS.tech.en.13.1.2649.2023
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