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13 (1) 2023

The phylogenetic authentication of Ophiocordyceps sphecocephala from Lang Biang Biosphere Reserve, Lam Dong, Vietnam


Author - Affiliation:
Lao Duc Thuan - Ho Chi Minh City Open University, Ho Chi Minh City , Vietnam
Pham Thi Dinh - Ho Chi Minh City Open University, Ho Chi Minh City , Vietnam
Thieu Hong Hue - Ho Chi Minh City Open University, Ho Chi Minh City , Vietnam
Vu Hoang Hai - Ho Chi Minh City Open University, Ho Chi Minh City , Vietnam
Nguyen Van Giang - Dalat University, Dalat City , Vietnam
Truong Binh Nguyen - Dalat University, Dalat City , Vietnam
Le Huyen Ai Thuy - Ho Chi Minh City Open University, Ho Chi Minh City , Vietnam
Corresponding author: Lao Duc Thuan - thuan.ld@ou.edu.vn
Submitted: 07-02-2023
Accepted: 03-03-2023
Published: 05-04-2023

Abstract
During our expedition at Lang Biang Biosphere Reserve, samples of DL0023, parasitizing on wasps, were collected, then sent to the biology laboratory, Dalat University, Lam Dong Province, Vietnam, to preliminarily morphologically identify the name of this species as Ophiocordyceps sphecocephala. The method of Phenol (pH = 8)/Chloroform method was applied to isolated DNA. The PCR assays and sequencing were performed to amplify and sequencing ITS, nrLSU, and nrSSU, respectively. The phylogenetic methods of neighbor-joining, maximum likelihood, and maximum parsimony were constructed to identify the relationship between DL0023 and referent sequences. We successfully amplified and sequenced the target genes, and constructed concatenated data containing 29, 33, and 30 sequences of ITS, nrLSU and nrSSU, respectively, belonging to Clavicipitaceae clade A, clade B and clade C, and 02 sequences belonged to Glomerellaceae. The results of phylogenetic analysis shown that DL0023 was accepted at Ophiocordyceps and strongly formed the monophyletic group Ophiocordyceps sphecocephala with a high supported value. In conclusion, the concatenated dataset’s phylogenetic analyses effectively supported the identification of DL0023 as Ophiocordyceps sphecocephala.

Keywords
entomopathogenic fungi; Lang Biang Biosphere Reserve; Ophiocordyceps; phylogeny

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Lao, T. D., Pham, D. T., Thieu, H. H., Vu, H. H., Nguyen, G. V., Truong, N. B., & Le, T. H. A. (2023). The phylogenetic authentication of Ophiocordyceps sphecocephala from Lang Biang Biosphere Reserve, Lam Dong, Vietnam. Ho Chi Minh City Open University Journal of Science – Engineering and Technology, 13(1), 18-23. doi:10.46223/HCMCOUJS.tech.en.13.1.2641.2023


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DOI: https://doi.org/10.46223/HCMCOUJS.tech.en.13.1.2641.2023
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