Real-time PCR application in confirmation test of Salmonella Typhimurium on instant noodle

Authors

  • Alfi Sophian
    National Center for Drug and Food Testing Development, Indonesia
    https://orcid.org/0000-0002-5206-2110
  • Sri Utaminingsih
    National Center for Drug and Food Testing Development, Indonesia
  • Tirta Setya Bhakti
    Center for Drug and Food Control in Semarang, Indonesia
  • Dewi Rahmawati
    Center for Drug and Food Control in Banjarmasin, Indonesia

DOI:

10.46223/HCMCOUJS.tech.en.13.1.2479.2023

Keywords:

bacteria; DNA; PCR; salmonella; testing

Abstract

Real-Time PCR is a molecular biology testing instrument used for various types of food product testing applications to monitor the quality of a product. In addition, the real-time PCR technique can also be used to test the detection of pathogenic bacteria in some foods and processed products. The purpose of this study was to detect Salmonella typhimurium using real-time PCR. This research can be a source of information and a reference for similar research. The test method used is the real-time PCR method with the green SYBR kit. Data analysis was carried out by looking at the results of interpreting Ct values and Tm values. Positive results are indicated by the formation of the Ct curve and the Tm curve. From the research conducted, it was found that the Ct value was in the range of 11.90 - 12.10 with an average of 12.02, while the Tm value was in the range of 85.10 - 85.20 with an average of 85.15. From the study results, it can be concluded that the use of real-time PCR for testing the detection of Salmonella typhimurium in instant noodle products can be applied.

Downloads

Download data is not yet available.

References

Beutin, L., Jahn, S., & Fach, P. (2009). Evaluation of the ‘GeneDisc’ real-time PCR system for detection of enterohaemorrhagic Escherichia coli (EHEC) O26, O103, O111, O145 and O157 strains according to their virulence markers and their O- and H-antigen-associated genes. Journal of Applied Microbiology, 106(4), 1122-1132.

Carlin, C. R., Lau, S. S., Cheng, R. A., Buehler, A. J., Kassaify, Z., & Wiedmann, M. (2020). Validation using diverse, difficult-to-detect salmonella strains and a dark chocolate matrix highlights the critical role of strain selection for evaluation of simplified, rapid PCR-based methods offering next-day time to results. Journal of Food Protection, 83(8), 1374-1386.

Ding, T., Suo, Y., Zhang, Z., Liu, D., Ye, X., Chen, S., & Zhao, Y. (2017). A multiplex RT-PCR assay for S. aureus, L. monocytogenes, and Salmonella spp. Detection in raw milk with pre-enrichment. Frontiers in Microbiology, 8, 1-11.

Dwight, Z., Palais, R., & Wittwer, C. T. (2011). uMELT: Prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web application. Bioinformatics, 27(7), 1019-1020.

Fukushima, H., Katsube, K., Tsunomori, Y., Kishi, R., Atsuta, J., & Akiba, Y. (2009). Comprehensive and rapid real-time PCR analysis of 21 foodborne outbreaks. International Journal of Microbiology, 2009, 1-13.

Downloads

Received: 24-09-2022
Accepted: 11-01-2023
Published: 05-04-2023

Statistics Views

Abstract: 735
PDF: 473
Crossref
Scopus
Google Scholar
Europe PMC

How to Cite

Sophian, A., Utaminingsih, S., Bhakti, T. S., & Rahmawati, D. (2023). Real-time PCR application in confirmation test of Salmonella Typhimurium on instant noodle. HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY, 13(1), 3–9. https://doi.org/10.46223/HCMCOUJS.tech.en.13.1.2479.2023

License

Copyright (c) 2023 Alfi Sophian; Sri Utaminingsih; Tirta Setya Bhakti; Dewi Rahmawati

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.