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12 (2) 2022

A multi locus sequence analysis scheme for phylogeny of the Bacillus subtilis species complex and its advantages over 16S rRNA genes


Author - Affiliation:
Dao Nu Dieu Hong - Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City , Vietnam
Trang Hoang Long - International University, Vietnam National University-HCMC , Vietnam
Nguyen Thi Thuy Tien - Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City , Vietnam
Dinh Anh Hoa - Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City , Vietnam
Tran Thi Phan - Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City , Vietnam
Le Thi Huynh Tram - Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City International University, Vietnam National University-HCMC , Vietnam
Corresponding author: Dao Nu Dieu Hong - dieuhong1791@gmail.com
Submitted: 25-04-2022
Accepted: 27-05-2022
Published: 04-11-2022

Abstract
A multi locus sequence analysis (MLSA approach) was studied on the Bacillus genus, or the Bacillus subtilis species complex for specific, including 08 strains from four species (B. subtilis, B. pumilus, B. licheniformis and B. amyloliquefaciens) were provided by Biotechnology Center of Ho Chi Minh City. The research was based on sequences of 16S rRNA genes, the concatenation of five protein-coding housekeeping genes: glpF, pta, purH, pycA, and rpoD. After PCR amplification and sequencing the phylogenetic tree of 16S rRNA sequences, concatenate sequences (as well as the phylogenetic tree of each housekeeping gene) are constructed for comparison and discussion. The aim of this study is reach for better resolution and differentiation of strains and species within the B. subtilis species and to determine whether MLSA scheme show advantages in 16S rRNA gene-based studies.

Keywords
a Multi Locus Sequence Analysis (MLSA approach); Bacillus subtilis; housekeeping genes; 16S rRNA

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Cite this paper as:

Dao, H. N. D., Trang, L. H., Nguyen, T. T. T., Dinh, H. A., Tran, P. T., & Le, T. T. H. (2022). A multi locus sequence analysis scheme for phylogeny of the Bacillus subtilis species complex and its advantages over 16S rRNA genes. Ho Chi Minh City Open University Journal of Science – Engineering and Technology, 12(2), 22-33. doi:10.46223/HCMCOUJS.tech.en.12.2.2255.2022


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DOI: https://doi.org/10.46223/HCMCOUJS.tech.en.12.2.2255.2022
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