A multi locus sequence analysis scheme for phylogeny of the Bacillus subtilis species complex and its advantages over 16S rRNA genes

Authors

  • Dao Nu Dieu Hong
    Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City, VN
  • Trang Hoang Long
    International University, Vietnam National University-HCMC, VN
  • Nguyen Thi Thuy Tien
    Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City, VN
  • Dinh Anh Hoa
    Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City, VN
  • Tran Thi Phan
    Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City, VN
  • Le Thi Huynh Tram
    Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City International University, Vietnam National University-HCMC, VN

DOI:

10.46223/HCMCOUJS.tech.en.12.2.2255.2022

Keywords:

a Multi Locus Sequence Analysis (MLSA approach); Bacillus subtilis; housekeeping genes; 16S rRNA

Abstract

A multi locus sequence analysis (MLSA approach) was studied on the Bacillus genus, or the Bacillus subtilis species complex for specific, including 08 strains from four species (B. subtilis, B. pumilus, B. licheniformis and B. amyloliquefaciens) were provided by Biotechnology Center of Ho Chi Minh City. The research was based on sequences of 16S rRNA genes, the concatenation of five protein-coding housekeeping genes: glpF, pta, purH, pycA, and rpoD. After PCR amplification and sequencing the phylogenetic tree of 16S rRNA sequences, concatenate sequences (as well as the phylogenetic tree of each housekeeping gene) are constructed for comparison and discussion. The aim of this study is reach for better resolution and differentiation of strains and species within the B. subtilis species and to determine whether MLSA scheme show advantages in 16S rRNA gene-based studies.

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References

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Additional Files

Received: 25-04-2022
Accepted: 27-05-2022
Published: 04-11-2022

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How to Cite

Hong, D. N. D., Long, T. H., Tien, N. T. T., Hoa, D. A., Phan, T. T., & Tram, L. T. H. (2022). A multi locus sequence analysis scheme for phylogeny of the Bacillus subtilis species complex and its advantages over 16S rRNA genes. HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY, 12(2), 22–33. https://doi.org/10.46223/HCMCOUJS.tech.en.12.2.2255.2022

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Copyright (c) 2022 Dao Nu Dieu Hong; Trang Hoang Long; Nguyen Thi Thuy Tien; Dinh Anh Hoa; Tran Thi Phan; Le Thi Huynh Tram

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