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12 (1) 2022

Cytotoxic activity related to survivin mRNA levels by Combretum quadrangulare Kurz extract against liver and breast cancer cells


Author - Affiliation:
Nguyen Thi Phuong - NTT Hi-tech Institute, Nguyen Tat Thanh University , Vietnam
Duong Thuc Huy - Department of Chemistry, Ho Chi Minh City University of Education , Vietnam
Nguyen Huu Hung - Faculty of Technology, Van Lang University , Vietnam
Corresponding author: Nguyen Thi Phuong - nguyenphuong@ntt.edu.vn
Submitted: 12-07-2021
Accepted: 18-08-2021
Published: 08-12-2021

Abstract
Liver cancer and breast cancer have high death rate and increased rapidly in recent years in Vietnam and over the world. Currently, the study of bioactive compounds isolated from plants for killing cancer cells is of interest. Herein, the study aims to identify the cytotoxicity using MTT assay of Combretum quadrangulare Kurz fractions against MCF-7 cells. RT-qPCR method was used to determine the survivin mRNA levels in P12 fraction-treated HepG2 and MCF-7 in comparison to DMSO-treated cells. The results showed that P12 fraction with IC50 of 20.9 ± 0.8 µg/mL was the best cytotoxicity on MCF-7 cells amongst P fractions. P12 fraction exhibited the apoptotic effect on HepG2 and MCF-7 cells by down-regulating survivin expression levels. Thus, P12 fraction was promising for the next research for the impact of fractions assessment in vivo or bioactive compounds isolation.

Keywords
apoptosis; breast cancer; Combretum quadrangulare; cytotoxicity; liver cancer; survivin

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Cite this paper as:

Nguyen, T. P., Duong, T. H. & Nguyen, H. H. (2022). Cytotoxic activity related to survivin mRNA levels by Combretum quadrangulare Kurz extract against liver and breast cancer cells. Ho Chi Minh City Open University Journal of Science – Engineering and Technology, 12(1), 55-64. doi:10.46223/HCMCOUJS. tech.en.12.1.1992.2022 


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