--

12 (1) 2022

Cytotoxic activity related to survivin mRNA levels by Combretum quadrangulare Kurz extract against liver and breast cancer cells


Author - Affiliation:
Nguyen Thi Phuong - NTT Hi-tech Institute, Nguyen Tat Thanh University , Vietnam
Duong Thuc Huy - Department of Chemistry, Ho Chi Minh City University of Education , Vietnam
Nguyen Huu Hung - Faculty of Technology, Van Lang University , Vietnam
Corresponding author: Nguyen Thi Phuong - nguyenphuong@ntt.edu.vn
Submitted: 12-07-2021
Accepted: 18-10-2021
Published: 08-12-2021

Abstract
Liver cancer and breast cancer have high death rate and increased rapidly in recent years in Vietnam and over the world. Currently, the study of bioactive compounds isolated from plants for killing cancer cells is of interest. Herein, the study aims to identify the cytotoxicity using MTT assay of Combretum quadrangulare Kurz fractions against MCF-7 cells. RT-qPCR method was used to determine the survivin mRNA levels in P12 fraction-treated HepG2 and MCF-7 in comparison to DMSO-treated cells. The results showed that P12 fraction with IC50 of 20.9 ± 0.8 µg/mL was the best cytotoxicity on MCF-7 cells amongst P fractions. P12 fraction exhibited the apoptotic effect on HepG2 and MCF-7 cells by down-regulating survivin expression levels. Thus, P12 fraction was promising for the next research for the impact of fractions assessment in vivo or bioactive compounds isolation.

Keywords
apoptosis; breast cancer; Combretum quadrangulare; cytotoxicity; liver cancer; survivin

Full Text:
PDF

Cite this paper as:

Nguyen, P. T., Duong, H. T. & Nguyen, H. H. (2022). Cytotoxic activity related to survivin mRNA levels by Combretum quadrangulare Kurz extract against liver and breast cancer cells. Ho Chi Minh City Open University Journal of Science – Engineering and Technology, 12(1), 55-64. doi:10.46223/HCMCOUJS.tech.en.12.1.1992.2022 


References

Adnyana, I. K., Tezuka, Y., Banskota, A. H., Tran, K. Q., & Kadota, S. (2001). Three new triterpenes from the seeds of Combretum quadrangulare and their hepatoprotective activity. Journal of Natural Products, 64(3), 360-363.


Al Dhaheri, Y., Eid, A., AbuQamar, S., Attoub, S., Khasawneh, M., Aiche, G., . . . Iratni, R. (2013). Mitotic arrest and apoptosis in breast cancer cells induced by Origanum majorana extract: Upregulation of TNF-α and downregulation of survivin and mutant p53. PloS One, 8(2), Article e56649.


Ambrosini, G., Adida, C., & Altieri, D. C. (1997). A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nature Medicine, 3(8), 917-921.


Banskota, A. H., Tezuka, Y., Adnyana, I. K., Xiong, Q., Hase, K., Tran, Q. K., . . . Kadota, S. (2000). Hepatoprotective effect of combretum quadrangulare and its constitutents. Biological and Pharmaceutical Bulletin, 23(4), 456-460.


Banskota, A. H., Tezuka, Y., Tran, Q. K., Saiki, I., Miwa, Y., Taga, T., & Kadota, S. (1998). Cytotoxic cycloartane-type triterpenes from Combretum quadrangulare. Bioorganic & Medicinal Chemistry Letters, 8(24), 3519-3524.


Banskota, A. H., Tezuka, Y., Tran, Q. K., Tanaka, K., Saiki, I., & Kadota, S. (2000a). Methyl quadrangularates AD and related triterpenes from Combretum quadrangulare. Chemical and Pharmaceutical Bulletin, 48(4), 496-504.


Banskota, A. H., Tezuka, Y., Tran, Q. K., Tanaka, K., Saiki, I., & Kadota, S. (2000b). Thirteen novel cycloartane-type triterpenes from Combretum quadrangulare. Journal of Natural Products, 63(1), 57-64.


Boykin, C., Zhang, G., Chen, Y., Zhang, R., Fan, X., Yang, W., & Lu, Q. (2011). Cucurbitacin IIa: A novel class of anti-cancer drug inducing non-reversible actin aggregation and inhibiting survivin independent of JAK2/STAT3 phosphorylation. British Journal of Cancer, 104(5), 781-789.


Bray, F., Ferlay, J., Soerjomataram, I., Siegel, R. L., Torre, L. A., & Jemal, A. (2018). Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal for Clinicians, 68(6), 394-424.


Chandele, A., Prasad, V., Jagtap, J. C., Shukla, R., & Shastry, P. R. (2004). Upregulation of survivin in G2/M cells and inhibition of caspase 9 activity enhances resistance in staurosporine-induced apoptosis. Neoplasia, 6(1), 29-40.


Chittasupho, C., & Athikomkulchai, S. (2018). Nanoparticles of Combretum quadrangulare leaf extract induce cytotoxicity, apoptosis, cell cycle arrest and anti-migration in lung cancer cells. Journal of Drug Delivery Science and Technology, 45(1), 378-387.


Conway, E. M., Pollefeyt, S., Steiner-Mosonyi, M., Luo, W., Devriese, A., Lupu, F., . . . Schaeffer, P. (2002). Deficiency of survivin in transgenic mice exacerbates Fas-induced apoptosis via mitochondrial pathways. Gastroenterology, 123(2), 619-631.


Eloff, J., Katerere, D., & McGaw, L. (2008). The biological activity and chemistry of the southern African Combretaceae. Journal of Ethnopharmacology, 119(3), 686-699.


Ghoncheh, M., Pournamdar, Z., & Salehiniya, H. (2016). Incidence and mortality and epidemiology of breast cancer in the world. Asian Pacific Journal of Cancer Prevention, 17(sup3), 43-46.


Kang, M. H., & Reynolds, C. P. (2009). Bcl-2 inhibitors: Targeting mitochondrial apoptotic pathways in cancer therapy. Clinical Cancer Research, 15(4), 1126-1132.


Li, F., Ambrosini, G., Chu, E. Y., Plescia, J., Tognin, S., Marchisio, P. C., & Altieri, D. C. (1998). Control of apoptosis and mitotic spindle checkpoint by survivin. Nature, 396(6711),
580-584.


Liu, Q., Fu, H., Xing, R., Tie, Y., Zhu, J., Sun, Z., & Zheng, X. (2008). Survivin knockdown combined with apoptin overexpression inhibits cell growth significantly. Cancer Biology & Therapy, 7(7), 1053-1060.


Livak, K. J., & Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods, 25(4), 402-408.


Nantachit, K., Sirilun, S., Nobsathian, S., & Rungjang, S. (2017). Three new polycyclic containing sulfur compounds from the seeds of Combretum quadrangulare kurz (Combretaceae), antifungal and anti-mycobacterium activities. Chiang Mai Journal of Science, 44(1), 157-167.


Nantachit, K., Tuchinda, P., Khantawa, B., & Roongjang, S. (2015). Antibacterial activity of extract and an isolated steroidal alkaloid from the seeds of combretum quadrangulare Kurz. Journal of Pharmacy and Pharmacology, 4(6), 261-267.


Narayan, V., Ravindra, K. C., Chiaro, C., Cary, D., Aggarwal, B. B., Henderson, A. J., & Prabhu, K. S. (2011). Celastrol inhibits Tat-mediated Human Immunodeficiency Virus (HIV) transcription and replication. Journal of Molecular Biology, 410(5), 972-983.


Nguyen, H. H., Nguyen, P. T., Nguyen, T. T., Phan, T. C., Dao, N. T. B., Dinh, M. T., . . . Duong, H. T. (2021). Two new cycloartanes from the leaves of Combretum quadrangulare growing in Vietnam and their biological activities. Arabian Journal of Chemistry, 14(7), Article 103189.


Nopsiri, W., Chansakaow, S., Putiyanan, S., Natakankitkul, S., & Santiarworn, D. (2014). Antioxidant and anticancer activities from leaf extracts of four Combretum species from Northern Thailand. Chiang Mai University Journal of Natural Sciences, 13(2), 195-205.


Printz, C. (2021). Female breast cancer most commonly diagnosed cancer globally. Cancer, 127(12), 1952-1953.


Roy, R., Singh, R. K., Jash, S. K., Sarkar, A., & Gorai, D. (2014). Combretum quadrangulare (Combretaceae): Phytochemical constituents and biological activity. Indo American Journal of Pharmaceutical Research, 4(8), 3416-3430.


Somanabandhu, A., Wungchinda, S., & Wiwat, C. (1980). Chemical composition of combretum quadrangulare Kurz. Paper presented at the 4th Asian Symposium on Medicinal Plants and Spices, Bangkok, Thailand.


Sung, H., Ferlay, J., Siegel, R. L., Laversanne, M., Soerjomataram, I., Jemal, A., & Bray, F. (2021). Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal for Clinicians, 71(3), 209-249.


Tamm, I., Wang, Y., Sausville, E., Scudiero, D. A., Vigna, N., Oltersdorf, T., & Reed, J. C. (1998). IAP-family protein survivin inhibits caspase activity and apoptosis induced by Fas (CD95), Bax, caspases, and anticancer drugs. Cancer Research, 58(23), 5315-5320.


Toume, K., Nakazawa, T., Ohtsuki, T., Arai, M. A., Koyano, T., Kowithayakorn, T., & Ishibashi, M. (2011). Cycloartane triterpenes isolated from Combretum quadrangulare in a screening program for death-receptor expression enhancing activity. Journal of Natural Products, 74(2), 249-255.


Vazquez, A., Bond, E. E., Levine, A. J., & Bond, G. L. (2008). The genetics of the p53 pathway, apoptosis and cancer therapy. Nature Reviews Drug Discovery, 7(12), 979-987.



DOI: https://doi.org/10.46223/HCMCOUJS.tech.en.12.1.1992.2022
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.